PLD1 antibody

Catalog No. ABIN498163

This Rabbit Polyclonal antibody specifically detects PLD1 in IHC (p). It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for PLD1 antibody (ABIN498163)

Target: PLD1 (Phospholipase D1 (PLD1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PLD1 antibody is un-conjugated

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-PLD1 Antibody

Specificity: This antibody detects endogenous levels of PLD1 protein.

Cross-Reactivity (Details): Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human.

Purification: Affinity chromatography using epitope-specific immunogen

Purity: > 95 % (by SDS-PAGE)

Application Details

Application Notes: Immunohistochemistry on paraffin sections: 1: 50 - 1: 200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Phosphate buffered saline (PBS) with 0.05 % sodium azide, approx. pH 7.2

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C for longer.

Target Details for PLD1

Target: PLD1 (Phospholipase D1 (PLD1))

Alternative Name: Phospholipase D1 / PLD1

Background: Activation of phosphatidylcholine-specific phospholipase D (PC-PLD) catalyzes the hydrolysis of phosphatidylcholine (PC) to generate phosphatidic acid (PA). Insulin activates the PLD-dependent hydrolysis of PC in plasma membranes of adipocytes by a mechanism that may involve wortmannin-sensitive phosphatidylinositol 3-kinase. In addition to the transient activation by growth factors stimulation, PC-PLD is constitutively activated in some of the Src- and Ras-transformed cells. PC-PLD is one of the target enzymes of ischemia, its decrease may cause a perturbation of PC hydrolysis and/or disorders of intracellular signal transduction or choline metabolism for acetylcholine formation in the brain.Synonyms: Choline phosphatase 1, PLD-1, Phosphatidylcholine-hydrolyzing phospholipase D1

Gene ID: 5337

NCBI Accession: NP_002653

UniProt: Q13393