PLCB3 antibody

Catalog No. ABIN498179

The Rabbit Polyclonal anti-PLCB3 antibody is suitable to detect PLCB3 in samples from Human, Rat and Mouse. It has been validated for WB and IHC (p).

Quick Overview for PLCB3 antibody (ABIN498179)

Target: PLCB3 (phospholipase C, beta 3 (Phosphatidylinositol-Specific) (PLCB3))

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PLCB3 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-PLCB3 Antibody

Specificity: This antibody detects endogenous levels of PLC β3 protein.

Cross-Reactivity (Details): Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human.

Purification: Affinity chromatography using epitope-specific immunogen

Purity: > 95 % (by SDS-PAGE)

Application Details

Application Notes: Western blot: 1: 500 - 1: 1000. Immunohistochemistry on paraffin sections: 1: 50 - 1: 200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Phosphate buffered saline (PBS) with 0.05 % sodium azide, approx. pH 7.2

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C for longer.

Target Details for PLCB3

Target: PLCB3 (phospholipase C, beta 3 (Phosphatidylinositol-Specific) (PLCB3))

Alternative Name: PLCB3

Background: Phosphoinositide-specific phospholipase C (PLC) plays a critical role in the initiation of receptor mediated signal transduction through the generation of the two second messengers, inositol 1, 4, 5-triphosphate and diacylglycerol from phosphatidylinositol 4, 5 bisphosphate. A total of eight mammalian PLC isozymes have been described (PLC β1, PLC β2, PLC β3, PLC β4, PLC γ1, PLC γ2, PLC δ1 and PLC δ2) with molecular weights ranging from 85 to 150 kDa. The γ-type enzymes are unique in that they contain SH2 and SH3 domains. Moreover, the two γ-type enzymes, but not the β and δ isozymes, are subject to activation by a number of protein tyrosine kinases which associate with their SH2 domains and induce their activation by phosphoryation. In contrast, activation of PLC β1, PLC β2 and PLC β3 is mediated by the α subunits of the Gq class of heterotrimeric G proteins and by certain βγ G protein subunits. The regulatory mechanisms for PLC δ1 and PLC δ2 are as yet not resolved.Synonyms: 1-phosphatidylinositol-4, 5-bisphosphate phosphodiesterase beta-3, PLC-beta-3, Phosphoinositide phospholipase C-beta-3, Phospholipase C-beta-3

Molecular Weight: approx. 150 kDa

Gene ID: 5331

NCBI Accession: NP_000923

UniProt: Q01970

Pathways: WNT Signaling, Thyroid Hormone Synthesis, Myometrial Relaxation and Contraction, CXCR4-mediated Signaling Events, G-protein mediated Events