SNAIL antibody

Catalog No. ABIN498268

This Rabbit Polyclonal antibody specifically detects SNAIL in WB, IF and IHC (p). It exhibits reactivity toward Human and Mouse and has been mentioned in 6+ publications.

Quick Overview for SNAIL antibody (ABIN498268)

Target: SNAIL (SNAI1)

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SNAIL antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-SNAIL Antibody

Specificity: This antibody detects endogenous levels of SNAI 1 protein. (region surrounding Ala242)

Cross-Reactivity (Details): Species reactivity (expected):Mouse, Rat.
Species reactivity (tested):Human.

Purification: Affinity Chromatography using epitope-specific immunogen.

Application Details

Application Notes: Western blot: 1: 500 - 1: 1000. Immunohistochemistry on paraffin sections: 1: 50 - 1: 200. Immunoflourescence: 1: 50 - 1: 200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.2, 0.05 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

References for anti-SNAIL antibody (ABIN498268)

Huang, Yu, Fu, Chen, Liu, Li, Xia, Zhang, Li: "EGFR inhibition prevents in vitro tumor growth of salivary adenoid cystic carcinoma." in: BMC cell biology, Vol. 14, pp. 13, (2013) (PubMed).

Yao, Li, Xiong, Li, Jiang, Chen: "A novel role of ribonuclease inhibitor in regulation of epithelial-to-mesenchymal transition and ILK signaling pathway in bladder cancer cells." in: Cell and tissue research, Vol. 353, Issue 3, pp. 409-23, (2013) (PubMed).

Pan, Xiong, Yao, Xin, Zhang, Chen: "Up-regulating ribonuclease inhibitor inhibited epithelial-to-mesenchymal transition and metastasis in murine melanoma cells." in: The international journal of biochemistry & cell biology, Vol. 44, Issue 6, pp. 998-1008, (2012) (PubMed).

Zou, Luo, Zeng, Dong, Wu, Liu: "Protein kinase CK2? is overexpressed in colorectal cancer and modulates cell proliferation and invasion via regulating EMT-related genes." in: Journal of translational medicine, Vol. 9, pp. 97, (2011) (PubMed).

Yang, Feng, Zhou, Liang, Mao, Deng, Yan, Sun, Du, Liu, Wang, Neckenig, Yang, Qu: "Aberrant expression of chemokine receptor CCR4 in human gastric cancer contributes to tumor-induced immunosuppression." in: Cancer science, Vol. 102, Issue 7, pp. 1264-71, (2011) (PubMed).

Feng, Di, Tao, Chang, Lu, Fan, Shan, Li, Yang: "PDK1 regulates vascular remodeling and promotes epithelial-mesenchymal transition in cardiac development." in: Molecular and cellular biology, Vol. 30, Issue 14, pp. 3711-21, (2010) (PubMed).

Target Details for SNAIL

Target: SNAIL (SNAI1)

Alternative Name: SNAI1

Background: The SNAIL family of developmental regulatory proteins is a group of widely conserved zinc-finger proteins that regulate transcription and include the mammalian proteins SLUG, SNAI 1, the human homolog of Drosophila SNAIL, and Smuc. SNAI 1 and SLUG are expressed in placenta and adult heart, liver and skeletal muscle. SNAI 1, and the corresponding mouse homolog Sn,a each contain three classic zinc fingers and one atypical zinc finger, while SLUG contains five zinc finger regions and a transcriptional repression domain at the amino terminus, which enables SLUG to act as a negative regulator of gene expression. SLUG is implicated in the generation and migration of neural crest cells in human embryos and also contributes to limb bud development. In addition, SLUG also constitutes a cellular anti-apoptotic transcription factor that effectively prevents apoptosis in murine pro-B cells deprived of IL-3. The SNAIL-related gene from murine skeletal muscle cells, Smuc, is highly expressed in skeletal muscle and thymus and can, likewise, repress gene transcription. Smuc preferentially associates with CAGGTG and CACCTG E-box motifs (CANNTG) on DNA and involves the five putative DNA-binding zinc finger domains at the C-terminal region of Smuc.Synonyms: Protein snail homolog 1, SNAH, Zinc finger protein SNAI1

Molecular Weight: approx. 29 kDa

Gene ID: 6615

NCBI Accession: NP_005976

UniProt: O95863

Pathways: Negative Regulation of intrinsic apoptotic Signaling