ADK antibody

Catalog No. ABIN498289

This Rabbit Polyclonal antibody specifically detects ADK in WB and IF. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for ADK antibody (ABIN498289)

Target: ADK (Adenosine Kinase (ADK))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ADK antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF)

Product Details anti-ADK Antibody

Specificity: This antibody detects endogenous levels of ADK protein. (region surrounding Glu6)

Cross-Reactivity (Details): Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human.

Purification: Affinity chromatography

Purity: > 95 % by SDS-PAGE

Application Details

Application Notes: Western Blot: 1/500 - 1/1000. Immunofluorescence: 1/50 - 1/200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1,0 mg/mL

Buffer: Phosphate buffered saline (PBS) with 0.05 % sodium azide, approx. pH 7.2.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for ADK

Target: ADK (Adenosine Kinase (ADK))

Alternative Name: Adenosine Kinase (AK)

Background: Adenosine kinase (ATP:adenosine 5 prime phosphotransferase) is an abundant enzyme in mammalian tissues that catalyzes the transfer of the gamma phosphate from ATP to adenosine, thereby serving as a potentially important regulator of concentrations of both extracellular adenosine and intracellular adenine nucleotides. Adenosine has widespread effects on the cardiovascular, nervous, respiratory, and immune systems and inhibitors of ADK could play an important pharmacological role in increasing intravascular adenosine concentrations and acting as antiinflammatory agents. The encoded protein does not present any sequence similarities to other well characterized mammalian nucleoside kinases. In contrast, 2 regions were identified with significant sequence identity to microbial ribokinase and fructokinases and a bacterial inosine/guanosine kinase. Thus, ADK is a structurally distinct mammalian nucleoside kinase that appears to be akin to sugar kinases of microbial origin.Synonyms: ADK, Adenosine 5'-phosphotransferase

Molecular Weight: approx. 40 kDa

Gene ID: 132

NCBI Accession: NP_001114

UniProt: P55263

Pathways: Ribonucleoside Biosynthetic Process