MAP4K4 antibody

Catalog No. ABIN498301

This Rabbit Polyclonal antibody specifically detects MAP4K4 in WB and IF. It exhibits reactivity toward Human and Mouse.

Quick Overview for MAP4K4 antibody (ABIN498301)

Target: MAP4K4 (Mitogen-Activated Protein Kinase Kinase Kinase Kinase 4 (MAP4K4))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MAP4K4 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF)

Product Details anti-MAP4K4 Antibody

Specificity: This antibody detects endogenous levels of HGK protein. (region surrounding Arg430)

Cross-Reactivity (Details): Species reactivity (expected):Mouse.
Species reactivity (tested):Human.

Purification: Affinity-chromatography using epitope-specific immunogen

Application Details

Application Notes: Western Blot: 1/500 - 1/1000. Immunohistochemistry: 1/50 - 1/200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.2., 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for MAP4K4

Target: MAP4K4 (Mitogen-Activated Protein Kinase Kinase Kinase Kinase 4 (MAP4K4))

Alternative Name: MAP4K4

Background: Several mammalian kinases have been identified which exhibit sequence similarity to the Saccharomyces cerevisiae serine/threonine kinase STE20. STE20 is involved in relaying signals from G-protein coupled receptors to cytosolic MAP kinase cascades, and it lies upstream of a MAP kinase kinase kinase. Mammalian STE20-like kinases include NIK, KHS, GLK, YSK1, HPK1, Krs-1, Krs-2, and GC kinase. NIK (Nck interacting kinase), like many of the STE20-like kinases, has been shown to activate the SAPK/JNK stress response pathway. Both the kinase domain and the C-terminal regulatory domain of NIK are required for full activation. NIK interacts with MEKK1 and is thought to act upstream of MEKK1 in the SAPK/JNK signaling pathway.Synonyms: HPK/GCK-like kinase HGK, MAPK/ERK kinase kinase kinase 4, MEKKK 4, Mitogen-activated protein kinase kinase kinase kinase 4, NIK, Nck-interacting kinase

Molecular Weight: approx. 142 kDa

Gene ID: 9448

NCBI Accession: NP_004825

UniProt: O95819