RBMX antibody

Catalog No. ABIN498314

Product Details anti-RBMX Antibody

Target: RBMX (RNA Binding Motif Protein, X-Linked (RBMX))

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RBMX antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Specificity: This antibody detects endogenous levels of hnRNP G protein. (region surrounding Met40)

Cross-Reactivity (Details): Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human.

Purification: Affinity-Chromatography using epitope-specific immunogen

Application Details

Application Notes: Western blot: 1: 500 - 1: 1000. Immunohistochemistry on paraffin sections: 1: 50 - 1: 200. Immunoflourescence: 1: 50 - 1: 200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS pH ~7.2, 0.05 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for RBMX

Target: RBMX (RNA Binding Motif Protein, X-Linked (RBMX))

Alternative Name: RBMX / HNRPG (RBMX Products)

Synonyms: hnRNP G antibody, HNRNPG antibody, HNRPG antibody, RBMXP1 antibody, RBMXRT antibody, RNMX antibody, hnRNP-G antibody, fc66e11 antibody, wu:fb37c07 antibody, wu:fc66e11 antibody, hnrnpg antibody, hnrpg antibody, rbmxp1 antibody, rbmxrt antibody, rnmx antibody, RBMXL1 antibody, RNA binding motif protein, X chromosome antibody, RNA binding motif protein, X-linked antibody, RNA binding motif protein, X-linked S homeolog antibody, Rbmx antibody, RBMX antibody, rbmx antibody, rbmx.S antibody

Background: Heterogeneous nuclear ribonucleoproteins (hnRNPs) constitute a set of polypeptides that contribute to mRNA transcription, pre-mRNA processing as well as mature mRNA transport to the cytoplasm and translation. They also bind heterogeneous nuclear RNA (hnRNA), which are the transcripts produced by RNA polymerase II. There are approximately 20 known hnRNP proteins, and their complexes are the major constituents of the spliceosome. The majority of hnRNP proteins components are localized to the nucleus, however some shuttle between the nucleus and the cytoplasm. hnRNP G is a glycoprotein, which was originally identified as an autoantigen from German shepherd dogs with lupus-like syndrome. The gene encoding hnRNP G is located on chromosome Xq26 and is ubiquitously expressed. It contains one RNP-consensus RNA binding domain (RBD) and is related to RBMY, which is involved in spermatogenesis, and hnRNP G-T, which is a testis specific protein. All three proteins interact with Tra2b and therefore, are involved in pre-mRNA splicing.Synonyms: Glycoprotein p43, Heterogeneous nuclear ribonucleoprotein G, RBMXP1, RNA-binding motif protein X chromosome, hnRNP G

Molecular Weight: approx. 40 kDa

Gene ID: 27316

NCBI Accession: NP_002130

UniProt: P38159

Pathways: Chromatin Binding, Ribonucleoprotein Complex Subunit Organization