CK1 epsilon antibody

Catalog No. ABIN498317

Product Details anti-CK1 epsilon Antibody

Target: CK1 epsilon (CSNK1E) (Casein Kinase 1, epsilon (CSNK1E))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CK1 epsilon antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Specificity: This antibody detects endogenous levels of Casein Kinase Iε protein. (region surrounding Pro301)

Cross-Reactivity (Details): Species reactivity (expected):Mouse.
Species reactivity (tested):Human.

Purification: Affinity chromatography using epitope-specific immunogen

Purity: > 95 % (by SDS-PAGE)

Application Details

Application Notes: Western blot: 1: 500 - 1: 1000. Immunohistochemistry on paraffin sections: 1: 50 - 1: 200. Immunoflourescence: 1: 50 - 1: 200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.2, 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C for longer.

Target Details for CK1 epsilon

Target: CK1 epsilon (CSNK1E) (Casein Kinase 1, epsilon (CSNK1E))

Alternative Name: Casein Kinase I epsilon (CSNK1E Products)

Synonyms: CKIepsilon antibody, HCKIE antibody, CKIe antibody, ck1-epsilon antibody, ck1epsilon antibody, hckie antibody, dco antibody, fj38c01 antibody, wu:fj38c01 antibody, wu:fj62a04 antibody, zgc:77310 antibody, AI426939 antibody, AI551861 antibody, AW457082 antibody, CK1epsilon antibody, KC1epsilon antibody, tau antibody, ckie antibody, CSNK1E antibody, Csnk1e antibody, CKI-epsilon antibody, 0538/13 antibody, 0915/10 antibody, 1396/02 antibody, 1447/01 antibody, 1460/09 antibody, CG2048 antibody, CK1delta/epsilon antibody, DBT antibody, DBT/CK1epsilon antibody, DCO antibody, DCO/CKIe antibody, Dbt antibody, Dco antibody, Dco/CK1 antibody, Dmel\CG2048 antibody, dCKIepsilon antibody, dbt antibody, dco-1 antibody, ddbt antibody, l(3)S053813 antibody, l(3)S144701 antibody, l(3)dco antibody, l(3)dco-1 antibody, l(3)discs overgrown antibody, l(3)discs overgrown-1 antibody, l(3)j3B9 antibody, l(3)rK215 antibody, casein kinase 1 epsilon antibody, casein kinase 1, epsilon antibody, casein kinase 1 epsilon L homeolog antibody, casein kinase I isoform epsilon antibody, casein kinase I epsilon antibody, casein kinase I antibody, LOC400927-CSNK1E readthrough antibody, discs overgrown antibody, CSNK1E antibody, csnk1e antibody, Csnk1e antibody, csnk1e.L antibody, LOC443240 antibody, NAEGRDRAFT_82890 antibody, LOC100407384 antibody, LOC100523244 antibody, LOC100607246 antibody, LOC100727500 antibody, LOC400927-CSNK1E antibody, dco antibody

Background: Casein kinase I (also designated CKI) and casein kinase II (also designated CKII) compose a family of serine/ threonine protein kinases which are present in all eukaryotes examined to date. CKI family members, which include CKIα, γ, ε and δ, have been implicated in the control of cytoplasmic and nuclear processes, including DNA replication and repair. CKII is usually expressed as a tetrameric complex consisting of either an α2β2 or an αα'β2 structure. The α catalytic subunit is stimulated by the β regulatory subunit, which undergoes autophosphorylation. CKII activity is high in the cytosol and nucleus of proliferating and differentiating cells. CKII is known to phosphorylate more than 100 different substrates including nuclear oncoproteins, transcription factors and enzymes involved in DNA metabolism.Synonyms: CKI-epsilon, CKIe, CSNK1E

Molecular Weight: approx. 47 kDa

Gene ID: 1454

NCBI Accession: NP_001885

UniProt: P49674

Pathways: Hedgehog Signaling, M Phase