LIMK2 antibody

Catalog No. ABIN498321

The Rabbit Polyclonal anti-LIMK2 antibody (ABIN498321) specifically detects LIMK2 in WB, IF and IHC (p). The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for LIMK2 antibody (ABIN498321)

Target: LIMK2 (LIM Domain Kinase 2 (LIMK2))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This LIMK2 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-LIMK2 Antibody

Specificity: This antibody detects endogenous levels of LIMK2 protein. (region surrounding Lys491)

Cross-Reactivity (Details): Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human.

Purification: Affinity-Chromatography using epitope-specific immunogen.

Application Details

Application Notes: Western Blot: 1/500-1/1000. Immunofluorescence: 1/50-1/200. Immunohistochemistry on Paraffin Sections: 1/50-1/200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Phosphate buffered saline (PBS), pH ~7.2 containing 0.05 % Sodium Azide as preservative.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for LIMK2

Target: LIMK2 (LIM Domain Kinase 2 (LIMK2))

Alternative Name: LIMK2

Background: Proteins containing LIM motifs are typically involved in cell fate determination and growth control. A family of proteins designated LIM kinases, incuding LIMK-1 and LIMK-2, has been identified. LIMK-1 has been shown to regulate the stabilization of F-Actin structures and Cofilin activity, indicating that LIMK-1 plays a role in a signaling pathway involved in the regulation of cell motility and morpho-genesis. LIMK-1 inhibits neuronal differentiation of PC12 cells, and it is thought to act by interfering with events downstream of MAPK activation. Expression patterns of LIMK-1 and LIMK-2 suggest that these proteins may have different functions during development. A truncated form of LIMK-2 has been identified in adult testis that is thought to arise from an alternative initiation exon.Synonyms: EC=2.7.11.1, LIM domain kinase 2, LIMK-2, LIMK2

Molecular Weight: approx. 72 kDa

Gene ID: 3985

NCBI Accession: NP_005560

UniProt: P53671