CSNK1G1 antibody

Catalog No. ABIN498359

The Rabbit Polyclonal anti-CSNK1G1 antibody is suitable to detect CSNK1G1 in samples from Human, Mouse and Rat. It has been validated for WB, IF and IHC (p).

Quick Overview for CSNK1G1 antibody (ABIN498359)

Target: CSNK1G1 (Casein Kinase 1, gamma 1 (CSNK1G1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CSNK1G1 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-CSNK1G1 Antibody

Specificity: This antibody detects endogenous levels of Casein Kinase Igamma1 protein. (region surrounding His3)

Cross-Reactivity (Details): Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human.

Purification: Affinity chromatography

Purity: > 95 % by SDS-PAGE

Application Details

Application Notes: Western Blot: 1/500 - 1/1000. Immunohistochemistry: 1/50 - 1/200. Immunofluorescence: 1/50 - 1/200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1,0 mg/mL

Buffer: Phosphate buffered saline (PBS) with 0.05 % sodium azide, approx. pH 7.2.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for CSNK1G1

Target: CSNK1G1 (Casein Kinase 1, gamma 1 (CSNK1G1))

Alternative Name: CSNK1G1

Background: Casein kinase I (also designated CKI) and casein kinase II (also designated CKII) compose a family of serine/ threonine protein kinases which are present in all eukaryotes examined to date. CKI family members, which include CKIalpha, gamma, epsilon and delta, have been implicated in the control of cytoplasmic and nuclear processes, including DNA replication and repair. CKII is usually expressed as a tetrameric complex consisting of either an alpha2-beta2 or an alpha-alpha'beta2 structure. The alpha catalytic subunit is stimulated by the beta regulatory subunit, which undergoes autophosphorylation. CKII activity is high in the cytosol and nucleus of proliferating and differentiating cells. CKII is known to phosphorylate more than 100 different substrates including nuclear oncoproteins, transcription factors and enzymes involved in DNA metabolism.Synonyms: CKI-gamma 1, Casein kinase I isoform gamma-1

Molecular Weight: approx. 49 kDa

Gene ID: 53944

NCBI Accession: NP_071331

UniProt: Q9HCP0

Pathways: Hedgehog Signaling