CIITA antibody

Catalog No. ABIN498419

The Rabbit Polyclonal anti-CIITA antibody is suitable to detect CIITA in samples from Human, Mouse and Rat. It has been validated for WB.

Quick Overview for CIITA antibody (ABIN498419)

Target: CIITA (Class II, Major Histocompatibility Complex, Transactivator (CIITA))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CIITA antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-CIITA Antibody

Specificity: This antibody detects endogenous levels of CIITA protein. (region surrounding Thr740)

Cross-Reactivity (Details): Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human.

Purification: Affinity chromatography

Purity: > 95 % by SDS-PAGE

Application Details

Application Notes: Western Blot: 1/500 - 1/1000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1,0 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.2., 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for CIITA

Target: CIITA (Class II, Major Histocompatibility Complex, Transactivator (CIITA))

Alternative Name: CIITA / C2TA

Background: The transactivator CIITA regulates basal and interferon-induced expression of MHC class II genes. CIITA restores expression of all MHC II gene expression in mutant cells and corrects regulatory defects of those genes. Activation is via the proximal promoter. No DNA binding of in vitro translated CIITA was detected. CIITA may act in a coactivator-like fashion through protein-protein interactions by contacting factors binding to the proximal MHC class II promoter, to elements of the transcription machinery, or both. Alternatively it may activate HLA class II transcription by modifying proteins that bind to the MHC class II promoter.Synonyms: MHC class II transactivator, MHC2TA

Molecular Weight: approx. 123 kDa

Gene ID: 4261

NCBI Accession: NP_000237

UniProt: P33076

Pathways: Cancer Immune Checkpoints