LAMa4 antibody

Catalog No. ABIN498447

The Rabbit Polyclonal anti-LAMa4 antibody (ABIN498447) specifically detects LAMa4 in WB, IF and IHC (p). The antibody is reactive with Human and Mouse samples.

Quick Overview for LAMa4 antibody (ABIN498447)

Target: LAMa4 (Laminin, alpha 4 (LAMa4))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This LAMa4 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-LAMa4 Antibody

Specificity: This antibody detects endogenous levels of Laminin alpha-4 protein. (region surrounding Ala511)

Cross-Reactivity (Details): Species reactivity (tested):Human and Mouse.

Purification: Affinity Chromatography using epitope-specific immunogen.

Application Details

Application Notes: Western Blot: 1/500-1/1000. Immunofluorescence: 1/50-1/200. Immunohistochemistry on Paraffin Sections: 1/50-1/200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.2, 0.05 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for LAMa4

Target: LAMa4 (Laminin, alpha 4 (LAMa4))

Alternative Name: Laminin alpha 4

Background: Laminins are essential and abundant structural non-collagenous glycoproteins localizing to basement membranes. Basement membranes (cellassociated extracellular matrices (ECMs)) are polymers of laminins with stabilizing type IV collagen networks, nidogen, and several proteoglycans.Basement membranes are found under epithelial layers, around the endothelium of blood vessels, and surrounding muscle, peripheral nerve, and fat cells. Formation of basement membranes influences cell proliferation, phenotype, migration, gene expression, and tissue architecture. Each laminin is a heterotrimer of α, β, and γ chain subunits that undergoes cell-secretion and incorporation into the ECM. Laminins can self-assemble, bind to other matrix macromolecules, and have unique and shared cell interactions mediated by integrins, dystroglycan, and cognate laminin receptors. The human laminin α-4 gene maps to chromosome 6q21 and is expressed in adult heart, lung, ovary, small and large intestines, liver, and placenta.Synonyms: LAMA4, Laminin subunit alpha-4

Molecular Weight: approx. 203 kDa

Gene ID: 3910

NCBI Accession: NP_001098676

UniProt: Q16363

Pathways: Brown Fat Cell Differentiation