BCL2L2 antibody

Catalog No. ABIN498448

This Rabbit Polyclonal antibody specifically detects BCL2L2 in WB, IF and IHC (p). It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for BCL2L2 antibody (ABIN498448)

Target: BCL2L2 (BCL2-Like 2 (BCL2L2))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This BCL2L2 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-BCL2L2 Antibody

Specificity: This antibody detects endogenous levels of Bcl-w protein. (region surrounding Gly154)

Cross-Reactivity (Details): Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human.

Purification: Affinity chromatography

Purity: > 95 % by SDS-PAGE

Application Details

Application Notes: Western Blot: 1/500 - 1/1000. Immunohistochemistry: 1/50 - 1/200. Immunofluorescence: 1/50 - 1/200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1,0 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.2., 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for BCL2L2

Target: BCL2L2 (BCL2-Like 2 (BCL2L2))

Alternative Name: Bcl-2-Like 2

Background: The Bcl-2 family of proteins is characterized by its ability to modulate cell death (apoptosis) under a broad range of physiological conditions. Bcl-2, A1 and Bcl-xL function to inhibit apoptosis while other members of the Bcl-2 family, Bax, Bad, Bak and Bcl-xS oppose their death-suppressing effects. Using a PCR-based strategy, an additional protein with life-promoting activity, designated Bcl-w, has been identified. The protein is highly conserved between mouse and human and is encoded by a gene located near the TCRalpha gene on chromosome 14. Bcl-w is expressed in myeloid cell lines but not in T and B lymphocytes, and can be found in a wide range of tissues. An alternative splicing event in exon 4 results in two transcripts. The first, Bcl-w, encodes a protein of 193 amino acids, and the second, Bcl-w/rox, encodes a protein 333 amino acids in length. The Synonyms: BCL2L2, BCLW, Bcl-w, Bcl2-L-2, KIAA0271

Molecular Weight: approx. 21 kDa

Gene ID: 599

NCBI Accession: NP_004041

UniProt: Q92843