PAX1 antibody

Catalog No. ABIN498514

Product Details anti-PAX1 Antibody

Target: PAX1 (Paired Box 1 (PAX1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PAX1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Specificity: This antibody detects endogenous levels of Pax-1 protein. (region surrounding Thr367)

Cross-Reactivity (Details): Species reactivity (tested):Human.

Purification: Affinity chromatography using epitope-specific immunogen

Purity: > 95 % (by SDS-PAGE)

Application Details

Application Notes: Western blot: 1: 500 - 1: 1000. Immunohistochemistry on paraffin sections: 1: 50 - 1: 200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.2, 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C for longer.

Target Details for PAX1

Target: PAX1 (Paired Box 1 (PAX1))

Alternative Name: PAX1 (PAX1 Products)

Synonyms: hup48 antibody, pax-1 antibody, HUP48 antibody, Pax-1 antibody, hbs antibody, hunchback antibody, un antibody, undulated antibody, wt antibody, paired box 1 antibody, paired box 1 S homeolog antibody, PAX1 antibody, pax1 antibody, Pax1 antibody, pax1.S antibody

Background: Pax genes contain paired domains with strong homology to genes in Drosophila that are involved in programming early development. Pax-1 is a sequence-specific DNA binding-protein with transcriptional activating properties. The expression pattern of Pax-1 during mouse embryogenesis indicates that it may play an important role in the development of the vertebral column. The autosomal recessive mutation 'undulated' (un) in the mouse exhibits vertebral anomalies along the entire rostrocaudal axis and is associated with a point mutation (G-to-A transition) at position 15 leading to a gly-to-ser replacement in a highly conserved region of the paired box of Pax-1. Pax-1 is required for the normal development of these three skeletal elements: the vertebral column, sternum, and scapula. Mice who are doubly heterozygous for the mutants 'undulated' and 'Patch' have a phenotype reminiscent of an extreme form of spina bifida occulta in humans. The gene which encodes Pax-1 maps to human chromosome 20p11.2.Synonyms: HUP48, Paired box protein Pax-1, Pax-1

Molecular Weight: approx. 36 kDa

Gene ID: 5075

NCBI Accession: NP_006183

UniProt: P15863