GJB1 antibody

Catalog No. ABIN498543

This anti-GJB1 antibody is a Rabbit Polyclonal antibody detecting GJB1 in WB and IHC (p). Suitable for Human, Rat and Mouse.

Quick Overview for GJB1 antibody (ABIN498543)

Target: GJB1 (Gap Junction Protein, beta 1, 32kDa (GJB1))

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GJB1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-GJB1 Antibody

Specificity: This antibody detects endogenous levels of Connexin-32 protein. (region surrounding Leu115)

Cross-Reactivity (Details): Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human.

Purification: Affinity Chromatography using epitope-specific immunogen

Application Details

Application Notes: Western blot: 1: 500 - 1: 1000. Immunohistochemistry on paraffin sections: 1: 50 - 1: 200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Phosphate buffered saline (PBS), pH ~7.2, 0.05 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for GJB1

Target: GJB1 (Gap Junction Protein, beta 1, 32kDa (GJB1))

Alternative Name: GJB1 / Cx32

Background: Gap junctions are aggregations of intercellular channels that directly connect the cytoplasm of adjacent cells. Gap junctions coordinate cellular and organ function in tissues and are involved in metabolic cooperation between cells, synchronization of cellular physiological activities, growth control and developmental regulation. The gap junction channels allow intercellular exchange of ions, nucleotides and small molecules between adjacent cells. Unlike other membrane channels, intercellular channels span two plasma membranes and require the contribution of hemichannels, called connexons, from both participating cells. These channels are permeate to molecules as large as 1 kD, and they have been detected in virtually every cell type in mammals, except mature skeletal muscle, spermatozoa and erythrocytes. Two connexons interact in the extracellular space to form the complete intercellular channel. Each connexon is composed of six similar or identical proteins, which are termed connexins. Connexins (Cx) are a multi-gene family of highly related proteins ranging between molecular weights of 26 and 70 kD. At least a dozen distinct connexin genes have been identified and many are expressed in a tissue-specific manner. Two distinct lineages have been identified in mammals. One termed class I or beta group to which Cx26, Cx30, Cx31, Cx31.1 and Cx32 belong, and the other termed class II or alpha group, represented by Cx33, Cx37, Cx40, Cx43 and Cx46.Synonyms: Connexin-32, GAP junction 28 kDa liver protein, Gap junction beta-1 protein

Molecular Weight: approx. 32 kDa

Gene ID: 2705

NCBI Accession: NP_000157

UniProt: P08034

Pathways: Cell-Cell Junction Organization