PGC antibody

Catalog No. ABIN498676

This Rabbit Polyclonal antibody specifically detects PGC in WB and IHC (p). It exhibits reactivity toward Human.

Quick Overview for PGC antibody (ABIN498676)

Target: PGC (Pepsinogen C (PGC))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PGC antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-PGC Antibody

Specificity: This antibody detects endogenous levels of Pepsin C protein. (region surrounding Phe306)

Cross-Reactivity (Details): Species reactivity (tested):Human.

Purification: Affinity chromatography

Purity: > 95 % by SDS-PAGE

Application Details

Application Notes: Western Blot: 1/500 - 1/1000. Immunohistochemistry: 1/50 - 1/200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1,0 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.2., 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for PGC

Target: PGC (Pepsinogen C (PGC))

Alternative Name: Pepsinogen Group II

Background: Pepsin is one of the main proteolytic enzymes secreted by the gastric mucosa. Pepsin consists of a single polypeptide chain and arises from its precursor, Pepsinogen, by removal of a 41 amino acid segment from the amino end. Pepsinogen is synthesized in the stomach lining, hydrochloric acid, also produced by the gastric mucosa, is necessary to convert the inactive enzyme and to maintain the optimum acidity ( pH 1-3) for Pepsin function. Pepsin is particularly effective in cleaving peptide bonds involving aromatic amino acids. It shows extremely broad specificity, and although bonds involving phenylalanine and leucine are preferred, many others are also cleaved to some extent. The amino acid composition of Pepsin C differs from those of Pepsinogen and Pepsin, especially in the content of basic amino acids, glutamic acid, aspartic acid, leucine and isoleucine.Synonyms: Gastricsin, PGC, Pepsinogen C, Progastricsin

Molecular Weight: approx. 42 kDa

Gene ID: 5225

NCBI Accession: NP_002621

UniProt: P20142