CAMK2A antibody (pThr286)

Catalog No. ABIN498777

The Rabbit Polyclonal anti-CAMK2A antibody (ABIN498777) specifically detects CAMK2A in WB and IHC (p). The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for CAMK2A antibody (pThr286) (ABIN498777)

Target: CAMK2A (Calcium/calmodulin-Dependent Protein Kinase II alpha (CAMK2A))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CAMK2A antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-CAMK2A Antibody

Binding Specificity: pThr286

Specificity: This antibody detects endogenous levels of p-CaMK2-a protein only when phosphorylated at Thr286.

Cross-Reactivity (Details): Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human.

Purification: Affinity chromatography

Purity: > 95 % by SDS-PAGE

Application Details

Application Notes: Western Blot: 1/500 - 1/1000. Immunohistochemistry: 1/50 - 1/200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1,0 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.2., 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for CAMK2A

Target: CAMK2A (Calcium/calmodulin-Dependent Protein Kinase II alpha (CAMK2A))

Alternative Name: CAMK2A

Background: The Ca2+/calmodulin-dependent protein kinases (CaM kinases) comprise a structurally related subfamily of serine/threonine kinases which include CaMKI, CaMKII and CaMKIV. CaMKII is an ubiquitously expressed serine/threonine protein kinase that is activated by Ca2+ and calmodulin (CaM) and has been implicated in regulation of the cell cycle and transcription. There are four CaMKII isozymes, designated alpha, beta, gamma and delta, which may or may not be co-expressed in the same tissue type. CaMKIIalpha is autophosphorylated on Thr 286 upon the binding of the Ca2+/CaM complex to the autoinhibitory domain of CaMKII. This process is called Ca2+/CaM trapping, which is thought to be involved in the synaptic encoding of information.Synonyms: CAMKA, CaM kinase II subunit alpha, CaM-kinase II alpha chain, CaMK-II subunit alpha, Calcium/calmodulin-dependent protein kinase type II alpha chain, KIAA0968

Molecular Weight: approx. 54 kDa

Gene ID: 815

NCBI Accession: NP_741960

UniProt: Q9UQM7

Pathways: WNT Signaling, Interferon-gamma Pathway, Myometrial Relaxation and Contraction