ERK1/2 antibody
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- Target See all ERK1/2 (MAPK1/3) Antibodies
- ERK1/2 (MAPK1/3) (Mitogen-Activated Protein Kinase 1/3 (MAPK1/3))
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Reactivity
- Human, Mouse, Rat
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This ERK1/2 antibody is un-conjugated
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Application
- Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- Cross-Reactivity (Details)
- Species reactivity (expected):Mouse and RatSpecies reactivity (tested):Human.
- Purification
- Affinity Chromatography using epitope-specific immunogen
- Top Product
- Discover our top product MAPK1/3 Primary Antibody
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- Application Notes
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Immunohistochemistry: 1/50 - 1/200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - Restrictions
- For Research Use only
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- Concentration
- 1.0 mg/mL
- Buffer
- Phosphate buffered saline (PBS), pH ~7.2, 0.05 % Sodium Azide
- Preservative
- Sodium azide
- Precaution of Use
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- Avoid repeated freezing and thawing.
- Storage
- 4 °C/-20 °C
- Storage Comment
- Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
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- Target
- ERK1/2 (MAPK1/3) (Mitogen-Activated Protein Kinase 1/3 (MAPK1/3))
- Alternative Name
- ERK1 / ERK2 (MAPK1/3 Products)
- Synonyms
- erk1/2 antibody, mitogen-activated protein kinase antibody, erk1/2 antibody
- Background
- The activation of signal transduction pathways by growth factors, hormones and neurotransmitters is mediated through two closely related MAP kinases, p44 and p42, designated extracellular-signal related kinase 1 (ERK 1) and ERK 2, respectively. ERK proteins are regulated by dual phosphorylation at specific tyrosine and Threonine sites mapping within a characteristic Thr- Glu-Tyr motif. Phosphorylation at both the Thr and Tyr residues is required for full enzymatic activation. In response to activation, MAP kinases phos-phorylate downstream components on Serine and Threonine. Upstream MAP kinase regulators include MAP kinase kinase (MEK), MEK kinase and Raf-1. The ERK family has three additional members: ERK 3, ERK 5 and ERK 6.Synonyms: ERK-1/ERK-2, Extracellular signal-regulated kinase, Insulin-stimulated MAP2 kinase, MAPK1/MAPK2, Mitogen-activated protein kinase, P42/P44-MAPK
- Molecular Weight
- approx. 42,44 kDa
- Gene ID
- 5595
- UniProt
- P27361
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