DAPK3 antibody

Catalog No. ABIN498992

The Rabbit Polyclonal anti-DAPK3 antibody is suitable to detect DAPK3 in samples from Human, Mouse and Rat. It has been validated for WB and IHC (p).

Quick Overview for DAPK3 antibody (ABIN498992)

Target: DAPK3 (Death-Associated Protein Kinase 3 (DAPK3))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DAPK3 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-DAPK3 Antibody

Specificity: This antibody detects endogenous levels of ZIPkinase protein. (region surrounding Lys261)

Cross-Reactivity (Details): Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human.

Purification: Affinity chromatography

Purity: > 95 % by SDS-PAGE

Application Details

Application Notes: Western Blot: 1/500 - 1/1000. Immunohistochemistry: 1/50 - 1/200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1,0 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.2., 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for DAPK3

Target: DAPK3 (Death-Associated Protein Kinase 3 (DAPK3))

Alternative Name: DAPK3 / ZIPK

Background: Apoptosis is mediated by death domain containing adapter molecules and a caspase family of proteases. Certainserine/threonine protein kinases, such as ASK1 and RIP, are mediators of apoptosis. A novel serine/threonine kinase that mediates apoptosis was recently identified and designated ZIP kinase. ZIP kinase contains an N terminal kinase domain and a C terminal leucine zipper structure and binds to ATF4 that is a member of ATF/CREBfamily. ZIP kinase has high sequence homology to DAP kinase (death associated protein kinase), which is a mediator of apoptosis induced by gamma interferon. Overexpression of ZIP kinase induces apoptosis. ZIP and DAP kinases represent a novel kinase family, which mediates apoptosis through their catalytic activities. The messenger RNA was ubiquitously expressed in various tissues.Synonyms: DAP kinase 3, DAP-like kinase, DLK, Death-associated protein kinase 3, ZIP-kinase

Molecular Weight: approx. 52 kDa

Gene ID: 1613

NCBI Accession: NP_001339

UniProt: O43293