C1S antibody

Catalog No. ABIN499051

This Rabbit Polyclonal antibody specifically detects C1S in WB. It exhibits reactivity toward Human.

Quick Overview for C1S antibody (ABIN499051)

Target: C1S (Complement Component 1, S Subcomponent (C1S))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This C1S antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-C1S Antibody

Specificity: This antibody detects endogenous levels of C1s protein. (region surrounding Lys583)

Cross-Reactivity (Details): Species reactivity (tested):Human.

Purification: Affinity chromatography

Purity: > 95 % by SDS-PAGE

Application Details

Application Notes: Western Blot: 1/500 - 1/1000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1,0 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.2., 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for C1S

Target: C1S (Complement Component 1, S Subcomponent (C1S))

Alternative Name: C1s

Background: C1s proenzyme is a glycoprotein produced primarily by hepatocytes but also by other cells. The proenzyme is produced as a single chain polypeptide of 688 aa. Upon activation the peptide is cleaved between R437 and I438 giving the activated enzyme consisting of two disulfide-linked chains, A chain (52.2 kDa) and B chain (27.7 kDa). The Ca dependent catalytic subunit of the C1 complex (C1q, C1r, C1s) comprises of two C1s and two C1r molecules and this serine protease tetramer is responsible for the initial steps in the classical complement pathway, cleavage of C4 and C2.Synonyms: C1 esterase, Complement 1s, Complement C1s, Complement C1s subcomponent, Complement component 1 subcomponent s

Molecular Weight: approx. 77 kDa

Gene ID: 716

NCBI Accession: NP_001725

UniProt: P09871

Pathways: Complement System