GRAP2 antibody

Catalog No. ABIN499081

This Rabbit Polyclonal antibody specifically detects GRAP2 in WB. It exhibits reactivity toward Human.

Quick Overview for GRAP2 antibody (ABIN499081)

Target: GRAP2 (GRB2-Related Adaptor Protein 2 (GRAP2))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GRAP2 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-GRAP2 Antibody

Specificity: This antibody detects endogenous levels of GRAP2 protein. (region surrounding asn158)

Cross-Reactivity (Details): Species reactivity (tested):Human.

Purification: Affinity chromatography using epitope-specific immunogen

Purity: > 95 % pure by SDS-PAGE

Application Details

Application Notes: Western blot: 1: 500 - 1: 1000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.2, 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C for longer.

Target Details for GRAP2

Target: GRAP2 (GRB2-Related Adaptor Protein 2 (GRAP2))

Alternative Name: GRAP2

Background: In general, it exists in association with catalytic domains, as in the nonreceptor protein-tyrosine kinases and phospholipase C-γ, within structural proteins, such as spectrin or myosin, and in small adapter proteins, such as Crk and GRB2. SH3 domains are often accompanied by SH2 domains of 100 amino acids that bind to tyrosine-phosphorylated regions of target proteins, frequently linking activated growth factors to putative signal transduction proteins. Deletion or mutation of SH3 domains generally activates the transforming potential of nonreceptor tyrosine kinases, suggesting that SH3 mediates negative regulation of an intrinsic transforming activity. Gads is an adapter proteins that contains both SH2 and SH3 domains. Gads binds to tyrosine-phosphorylated proteins, such as Shc, and functions to couple these proteins to downstream effectors.Synonyms: Adapter protein GRID, GADS, GRB-2-like protein, GRB2-related adapter protein 2, GRB2L, GRBLG, GRBX, GRID, Grf-40, Grf40 adapter protein, Growth factor receptor-binding protein, Hematopoietic cell-associated adapter protein GrpL, SH3-SH2-SH3 adapter Mona

Molecular Weight: approx. 38 kDa

Gene ID: 9402

NCBI Accession: NP_004801

UniProt: O75791

Pathways: TCR Signaling, Fc-epsilon Receptor Signaling Pathway