ETFB antibody (AA 231-243)

Catalog No. ABIN499132

This Goat Polyclonal antibody specifically detects ETFB in WB, IHC (p) and EIA. It exhibits reactivity toward Human, Mouse, Rat, Cow and Dog.

Quick Overview for ETFB antibody (AA 231-243) (ABIN499132)

Target: ETFB (Electron-Transfer-Flavoprotein, beta Polypeptide (ETFB))

Reactivity: Human, Mouse, Rat, Cow, Dog

Host: Goat

Clonality: Polyclonal

Conjugate: This ETFB antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Product Details anti-ETFB Antibody

Binding Specificity: AA 231-243

Sequence: C-PQRTAGVKVE TTE

Specificity: This antibody is expected to recognize both reported isoforms (NP_001976.1 and NP_001014763.1).

Cross-Reactivity (Details): Species reactivity (expected):Canine, Bovine.
Species reactivity (tested):Human, Mouse and Rat.

Purification: Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide.

Immunogen: Peptide with sequence from the C-terminalregion (aa 231-243) of the protein sequence according to NP_001976.1 and NP_001014763.1. Genename: ETFB

Application Details

Application Notes: Peptide ELISA: Limit Dilution: 1/8000. Western blot: 0.01-0.03 μg/mL. This antibody detects a band of approx. 26 kDa in Human, Mouseand Rat Liver lysates.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, pH 7.3 containing 0.02 % Sodium Azide as preservative and 0.5 % BSA as stabilizer.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for ETFB

Target: ETFB (Electron-Transfer-Flavoprotein, beta Polypeptide (ETFB))

Alternative Name: ETFB

Background: ETFB is a is an electron-transfer-flavoprotein, (beta polypeptide), and a subcomponent of Electron transfer flavoprotein (ETF). ETF exists in the mitochondrial matrix as a heterodimer of 30-kD alpha subunits (ETFA) and 28-kD beta subunits (ETFB) and contains 1 flavin adenine dinucleotide (FAD) and 1 adenosine 5-prime monophosphate (AMP) per heterodimer. This electron transfer flavoprotein serves as a specific electron acceptor for several dehydrogenases and transfers the electrons to the main mitochondrial respiratory chain via ETF-ubiquinone oxidoreductase (ETF dehydrogenase).Synonyms: Beta-ETF, Electron transfer flavoprotein subunit beta

Gene ID: 2109

NCBI Accession: NP_001014763

UniProt: P38117