beta Actin antibody (C-Term)

Catalog No. ABIN499441

This Rabbit Polyclonal antibody specifically detects beta Actin in WB, IF and EIA. It exhibits reactivity toward Human, Mouse and Ratand has been mentioned in 1 publication.

Quick Overview for beta Actin antibody (C-Term) (ABIN499441)

Target: beta Actin (ACTB) (Actin, beta (ACTB))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This beta Actin antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Enzyme Immunoassay (EIA)

Product Details anti-beta Actin Antibody

Binding Specificity: C-Term

Specificity: Beta-actin antibody was raised against a 16 amino acid peptide from near the carboxy-terminus of human beta-actin.

Purification: Affinity chromatography purified via peptide column

Immunogen: Human b-actin / Beta actin (C-Terminus) Peptide

Isotype: IgG

Application Details

Application Notes: ELISA. Western Blot: beta-actin antibody can be used for the detection of beta-actin at 1 - 2 μg/mL. Immnocytochemistry.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: PBS containing 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store the antibody undiluted at 2-8 °C.

References for anti-beta Actin antibody (ABIN499441)

Ghate, Hazra, Sarkar, Mandal: "Heartwood extract of Acacia catechu induces apoptosis in human breast carcinoma by altering bax/bcl-2 ratio." in: Pharmacognosy magazine, Vol. 10, Issue 37, pp. 27-33, (2014) (PubMed).

Target Details for beta Actin

Target: beta Actin (ACTB) (Actin, beta (ACTB))

Alternative Name: Actin beta / ACTB

Background: Actins are highly conserved proteins that are involved in cell motility, structure and integrity, processes that are crucial for tissue development and the development of organism (reviewed in 1). The actin cytoskeleton is one of the principal drivers of cell motility and is capable of responding to complex signaling cascades. Recent evidence suggests that it may play key roles in regulating apoptosis and aging (2). beta actin is one of six different actin isoforms which have been identified. Like GAPDH, Β-actin is constitutively expressed at high levels in almost all tissues and cell lines making it ideal for use as a loading control marker in immunoblots.Synonyms: Actin cytoplasmic 1, Beta-Actin

Gene ID: 60

UniProt: P60709

Pathways: Myometrial Relaxation and Contraction, Cell-Cell Junction Organization, Maintenance of Protein Location, Phototransduction