beta Actin antibody (N-Term)

Catalog No. ABIN499445

The Chicken Polyclonal anti-beta Actin antibody has been validated for WB, IF and EIA. It is suitable to detect beta Actin in samples from Human, Mouse and Rat.

Quick Overview for beta Actin antibody (N-Term) (ABIN499445)

Target: beta Actin (ACTB) (Actin, beta (ACTB))

Reactivity: Human, Mouse, Rat

Host: Chicken

Clonality: Polyclonal

Conjugate: This beta Actin antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Enzyme Immunoassay (EIA)

Product Details anti-beta Actin Antibody

Binding Specificity: N-Term

Specificity: This antibody reacts to Beta-Actin.

Purification: Affinity chromatography purified via peptide column

Immunogen: Beta-actin antibody was raised against a 16 amino acid peptide from near the amino terminus of human beta-actin.

Isotype: IgG

Application Details

Application Notes: ELISA. Western Blot: Beta-actin antibody can be used for the detection of beta-actin at 1 - 2 μg/mL. Immunocytochemistry.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Buffer: PBS containing 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store the antibody undiluted at 2-8 °C.

Target Details for beta Actin

Target: beta Actin (ACTB) (Actin, beta (ACTB))

Alternative Name: Actin beta / ACTB

Background: Actins are highly conserved proteins that are involved in cell motility, structure and integrity, processes that are crucial for tissue development and the development of organism. The actin cytoskeleton is one of the principal drivers of cell motility and is capable of responding to complex signaling cascades. Recent evidence suggests that it may play key roles in regulating apoptosis and aging. Beta actin is one of six different actin isoforms which have been identified. Like GAPDH, b-actin is constitutively expressed at high levels in almost all tissues and cell lines making it ideal for use as a loading control marker in immunoblots.Synonyms: Actin cytoplasmic 1, Beta-Actin

Gene ID: 60

UniProt: P60709

Pathways: Myometrial Relaxation and Contraction, Cell-Cell Junction Organization, Maintenance of Protein Location, Phototransduction