CARD8 antibody

Catalog No. ABIN499535

The Rabbit Polyclonal anti-CARD8 antibody has been validated for WB, IF and EIA. It is suitable to detect CARD8 in samples from Human.

Quick Overview for CARD8 antibody (ABIN499535)

Target: CARD8 (Caspase Recruitment Domain Family, Member 8 (CARD8))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CARD8 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Enzyme Immunoassay (EIA)

Product Details anti-CARD8 Antibody

Specificity: CARD8 antibody was raised against a synthetic peptide corresponding to amino acids at the C-terminus of human CARD8.

Purification: Affinity chromatography purified via peptide column

Immunogen: Human CARD8 / Cardinal / TUCAN Peptide

Isotype: IgG

Application Details

Application Notes: ELISA. Immunocytochemistry. Western Blot: CARD8 antibody can be used for detection of CARD8 at 2 μg/mL. K562 cell lysate can be used as Positive Control and a band at approximately 45 kDa canbe detected.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS containing 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store the antibody undiluted at 2-8 °C.

Target Details for CARD8

Target: CARD8 (Caspase Recruitment Domain Family, Member 8 (CARD8))

Alternative Name: CARD8 / NDPP1

Background: Apoptosis is related to many diseases and development. Cell death signals are transduced by death domain (DD), death effector domain (DED), and caspase recruitment domain (CARD) containing molecules. CARD containing proteins include some caspases, Apaf-1, CARD4, IAPs, RICK, ARC, RAIDD, BCL-10, and ASC. A novel CARD-containing protein was recently identified and designated CARD8. This protein interacts with DRAL, a p53-responsive protein implicated in the induction of apoptosis (1,2), and caspase-1 and its related proteins ICEBERG and pesudo-ICE (3). Although there are conflicting reports on whether CARD8 acts a pro- or anti-apoptotic protein (2,4), it has been suggested that it functions as an adaptor molecule regulating caspase-1 and NF-kappaB activation.Synonyms: Apoptotic protein NDPP-1, CARD-inhibitor of NF-kappa-B-activating ligand, CARDINAL, Caspase recruitment domain-containing protein 8, DACAR, KIAA0955, TUCAN, Tumor up-regulated CARD-containing antagonist of CASP9

Gene ID: 22900

UniProt: Q9Y2G2

Pathways: Positive Regulation of Endopeptidase Activity