Caspase 12 antibody (large)

Catalog No. ABIN499551

This anti-Caspase 12 antibody is a Rabbit Polyclonal antibody detecting Caspase 12 in WB, IHC (p) and EIA. Suitable for Human, Mouse and Rat.

Quick Overview for Caspase 12 antibody (large) (ABIN499551)

Target: Caspase 12 (CASP12) (Caspase 12 (Gene/pseudogene) (CASP12))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Caspase 12 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Product Details anti-Caspase 12 Antibody

Binding Specificity: large

Specificity: Caspase-12 antibody was raised against a synthetic peptide corresponding to amino acids within the large cleavage product of murine caspase-12.

Purification: Affinity chromatography purified via peptide column

Immunogen: Mouse Caspase-12 (Large) Peptide

Isotype: IgG

Application Details

Application Notes: ELISA. Western Blot: Caspase-12 (large) antibody can be used for the detection of both thepropeptide and cleaved forms of caspase-12 at 0.5 to 2 μg/mL. Immunohistochemistry.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Buffer: PBS containing 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store the antibody undiluted at 2-8 °C.

Target Details for Caspase 12

Target: Caspase 12 (CASP12) (Caspase 12 (Gene/pseudogene) (CASP12))

Alternative Name: Caspase-12

Background: Three distinct signaling pathways lead to programmed cell death (apoptosis). The death receptor and mitochondrion pathways are the main, in which the key apoptotic proteases capase-8 and caspase-9, respectively, are involved. The endoplasmic reticulum (ER) stress is the third apoptotic pathway and caspase-12 is involved (1,2). Caspase-12 is localized to the ER but not to cytoplasm or mitochondrion. Caspase-12 is activated by ER stress, including disruption of ER calcium homeostasis, and mediates ER stress-induced apoptosis. Caspase-12 is co-localized to the ER with several proteins that are involved in Alzheimer's disease including beta-secretase presenilin and beta-amyloid precursor protein (APP). Caspase-12 mediates cytotoxicity induced by amyloid-beta. Caspase-12 is ubiquitously expressed in mouse tissues (1).Synonyms: CASP-12, CASP12, Inactive caspase-12

Gene ID: 100044205

UniProt: O08736

Pathways: Apoptosis, ER-Nucleus Signaling, Positive Regulation of Endopeptidase Activity, Unfolded Protein Response