CBL antibody (C-Term)

Catalog No. ABIN499585

This Rabbit Polyclonal antibody specifically detects CBL in WB, IF and EIA. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for CBL antibody (C-Term) (ABIN499585)

Target: CBL (Cas-Br-M (Murine) Ecotropic Retroviral Transforming Sequence (CBL))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CBL antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Enzyme Immunoassay (EIA)

Product Details anti-CBL Antibody

Binding Specificity: C-Term

Specificity: This antibody reacts to CBL.

Purification: Affinity chromatography purified via peptide column

Immunogen: Cbl antibody was raised against a 14 amino acid peptide from near the carboxy terminus of human cbl.

Isotype: IgG

Application Details

Application Notes: ELISA. Western Blot: Cbl antibody can be used for detection of cbl at 0.5 - 1 μg/mL. Immunofluorescence.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Buffer: PBS containing 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store the antibody undiluted at 2-8 °C.

Target Details for CBL

Target: CBL (Cas-Br-M (Murine) Ecotropic Retroviral Transforming Sequence (CBL))

Alternative Name: CBL / RNF55

Background: The mammalian cbl family of ubiquitin ligases consists of three homologs known as cbl (also known as c-Cbl), Cbl-B, and Cbl-3 which share highly conserved a tyrosine-kinase-binding domain, linker and RING finger domain in their amino-terminal halves. Similar to other E3 ubiquitin ligases, Cbl catalyzes the transfer of ubiquitin from an E2 or Ubc (ubiquitin-conjugating) enzyme to the e-amino group of a lysine residue of the substrate protein. Cbl acts to negatively regulate many types of cell-surface receptors, including the Syk protein tyrosine kinase family. Cbl is thought to be involved in T- and B-cell signaling, in addition to thymus development. Of the three known homologs in the cbl family, cbl antibody reacts specifically with cbl. Multiple isoforms of cbl have been reported.Synonyms: CBL2, Casitas B-lineage lymphoma proto-oncogene, E3 ubiquitin-protein ligase CBL, Proto-oncogene c-CBL, RING finger protein 55, Signal transduction protein CBL, c-CBL

Gene ID: 867

UniProt: P22681

Pathways: TCR Signaling, Interferon-gamma Pathway, EGFR Signaling Pathway, EGFR Downregulation, VEGFR1 Specific Signals