CENPO antibody (Center)

Catalog No. ABIN499615

This Rabbit Polyclonal antibody specifically detects CENPO in WB and EIA. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for CENPO antibody (Center) (ABIN499615)

Target: CENPO (Centromere Protein O (CENPO))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CENPO antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Product Details anti-CENPO Antibody

Binding Specificity: Center

Purification: Affinity chromatography purified via peptide column

Immunogen: CENPO antibody was raised against a 16 amino acid peptide from near the center of human CENPO.

Isotype: IgG

Application Details

Application Notes: ELISA. Western Blot: CENPO antibody can be used for detection of CENPO at 1 - 2 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS containing 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

Storage Comment: Store the antibody (in aliquots) at -20 °C.

Target Details for CENPO

Target: CENPO (Centromere Protein O (CENPO))

Alternative Name: CENP-O

Background: Accurate chromosome segregation during mitosis requires a kinetochore to assemble correctly at the centromere of each chromatid and form a dynamic interface with the microtubules of the mitotic spindle. The kinetochore assembly includes the multisubunit CENP-H/I complex which can be divided into three functional classes based on phenotype analysis. One of these classes is referred to as the CENP-O class of proteins of which CENPO is the exemplar. These proteins form a stable complex with each other and are required for proper kinetochore function and for recovery from mitotic spindle damage. CENPO has also been shown to be an auto-antigen in a small population of anti-centromere antibodies- (ACA-) positive patients with sclerodoma.Synonyms: CENPO, Centromere protein O, ICEN36, Interphase centromere complex protein 36

Gene ID: 79172

NCBI Accession: NP_077298

UniProt: Q9BU64