APOE antibody (AA 151-250) (AbBy Fluor® 680)

Catalog No. ABIN4996735

The Rabbit Polyclonal anti-APOE antibody has been validated for WB, IF (p) and IF (cc). It is suitable to detect APOE in samples from Human, Mouse and Rat.

Quick Overview for APOE antibody (AA 151-250) (AbBy Fluor® 680) (ABIN4996735)

Target: APOE (Apolipoprotein E (APOE))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This APOE antibody is conjugated to AbBy Fluor® 680

Application: Western Blotting (WB), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunofluorescence (Cultured Cells) (IF (cc))

Product Details anti-APOE Antibody (AbBy Fluor® 680)

Binding Specificity: AA 151-250

Cross-Reactivity: Human, Mouse, Rat

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human APOE

Isotype: IgG

Application Details

Application Notes: IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for APOE

Target: APOE (Apolipoprotein E (APOE))

Alternative Name: Apolipoprotein E

Background:

Synonyms: AD2, LPG, APO-E, LDLCQ5, Apolipoprotein E, APOE, ApoE3, APOE-epsilon3, ApoE4, APOE-epsilon4,

Background: Apolipoprotein-E (apoE) is a protein component of plasma lipoproteins that mediates the binding, internalization and catabolism of lipoprotein particles. It can serve as a ligand for several lipoprotein receptors, including the LDL (ApoB/E) receptor and the hepatic apoE (chylomicron remnant) receptor. apoE is produced in most organs and occurs in all plasma lipoprotein fractions, constituting 10-20 % of VLDL (very low density lipoprotein) and 1-2 % of HDL (high density lipoprotein). Three major isoforms of apoE have been described in human (E2, E3 and E4) which differ by only one or two amino acids. Estrogen receptor has been shown to upregulate apoE gene expression via the ERa-mediated pathway, indicating a potential role for apoE in atherosclerosis. This is consistent with studies in mice in which plasma apoE levels were raised, thereby protecting the mice from diet-induced atherosclerosis. apoE has also been shown to be a potent inhibitor of proliferation and thus may play a role in angiogenesis, tumor cell growth and metastasis.

Gene ID: 348

UniProt: P02649

Pathways: Regulation of Cell Size, Lipid Metabolism