DDX3X antibody (Intermediate Domain)

Catalog No. ABIN499730

The Rabbit Polyclonal anti-DDX3X antibody is suitable to detect DDX3X in samples from Human, Mouse and Rat. It has been validated for WB, IF and EIA.

Quick Overview for DDX3X antibody (Intermediate Domain) (ABIN499730)

Target: DDX3X (DEAD (Asp-Glu-Ala-Asp) Box Polypeptide 3, X-Linked (DDX3X))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DDX3X antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Enzyme Immunoassay (EIA)

Product Details anti-DDX3X Antibody

Binding Specificity: Intermediate Domain

Purification: Affinity chromatography purified via peptide column

Immunogen: Human DDX3 (Intermediate Domain) Peptide

Isotype: IgG

Application Details

Application Notes: ELISA. Western BLot: 1 - 2 μg/mL. Immunocytochemistry.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Buffer: PBS containing 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store the antibody undiluted at 2-8 °C.

Target Details for DDX3X

Target: DDX3X (DEAD (Asp-Glu-Ala-Asp) Box Polypeptide 3, X-Linked (DDX3X))

Alternative Name: DDX3X

Background: DDX3 contains all of the motifs of the DEAD-box family of RNA helicases, including the Asp-Glu-Ala-Asp sequence that gives the protein family its name and distinguishes it from other RNA helicases (1,2). DDX3 is localized to the X chromosome and has a highly conserved functional homolog (DBY) on the Y chromosome (3). DDX3 is thought to be involved in RNA splicing, RNA transport, and translation initiation (4). It has also been shown to be involved in cell growth control and is deregulated in hepatitis virus-associated hepatocellular carcinoma (5). Recent experiments suppressing DDX3 expression blocked HIV-1 RNA export from the nucleus, suggesting that DDX3 functions as a shuttling protein that transports the HIV-1 protein Rev and its cofactor CRM1 from the nucleus to the cytoplasm (6).Synonyms: ATP-dependent RNA helicase DDX3X, DBX, DDX3, DEAD box X isoform, DEAD box protein 3 X-chromosomal, DEAD3, Embryonic RNA helicase, HLP2, Helicase-like protein 2

Gene ID: 1654

UniProt: O00571

Pathways: Ribonucleoprotein Complex Subunit Organization, Positive Regulation of Endopeptidase Activity, Negative Regulation of intrinsic apoptotic Signaling, Ribosome Assembly