EBI3 antibody (Center)

Catalog No. ABIN499778

The Rabbit Polyclonal anti-EBI3 antibody has been validated for WB, IHC (p) and EIA. It is suitable to detect EBI3 in samples from Human, Mouse and Rat.

Quick Overview for EBI3 antibody (Center) (ABIN499778)

Target: EBI3 (IL-27b) (Interleukin-27 subunit beta (IL-27b))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This EBI3 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Product Details anti-EBI3 Antibody

Binding Specificity: Center

Specificity: This antibody reacts to EBI3.

Purification: Affinity chromatography purified via peptide column

Immunogen: EBI3 antibody was raised against a 16 amino acid peptide from near the center of human EBI3.

Isotype: IgG

Application Details

Application Notes: ELISA. Western Blot: 2 - 4 μg/mL. Immunohistochemistry.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Buffer: PBS containing 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store the antibody undiluted at 2-8 °C.

Target Details for EBI3

Target: EBI3 (IL-27b) (Interleukin-27 subunit beta (IL-27b))

Alternative Name: EBI3 / IL27B

Target Type: Viral Protein

Background: EBI3 is a subunit in two distinct heterodimeric cytokines: interleukin-27 (IL-27) and IL-35 (1-3). Like interleukin-23 (IL-23), IL-27 is a recently discovered member of the IL-6/IL-12 family of proinflammatory and immunoregulatory cytokines. It exists as a heterodimer composed of the p40-related protein EBI3 and an IL-12 p35-related protein termed p28. IL-27 is produced after activation by antigen-presenting cells and induces proliferation of naive but not memory CD4+ T-cells. It acts by binding to its receptor WSX-1 (also known as TCCR) and gp130 which results in the activation of a Jak/STAT signaling cascade, suggesting the IL-27 is involved in the regulation of immune processes. It has been suggested that IL-27 can also be used as a therapeutic agent against cancer as it can also induce tumor-specific anti-tumor activity mediated through CD8+ T-cells, IFN-g, and T-bet. IL-35 is composed of EBI3 and the p35 subunit of IL-12 and has been reported to have therapeutic effects against collagen-induced arthritis by expanding the population of regulatory T cells and suppressing Th17 cells. At least two isoform of EBI3 are known to exist.Synonyms: EBV-induced gene 3 protein, Epstein-Barr virus-induced gene 3 protein, IL-27 subunit beta, IL-27B, Interleukin-27 subunit beta

Gene ID: 10148

NCBI Accession: NP_005746

UniProt: Q14213