Endonuclease G antibody

Catalog No. ABIN499796

The Rabbit Polyclonal anti-Endonuclease G antibody has been validated for WB, IHC (p) and EIA. It is suitable to detect Endonuclease G in samples from Human, Rat and Mouse.

Quick Overview for Endonuclease G antibody (ABIN499796)

Target: Endonuclease G (ENDOG)

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Endonuclease G antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Product Details anti-Endonuclease G Antibody

Specificity: EndoG antibody was raised with a synthetic peptide corresponding to 15 amino acids near the amino terminus of human EndoG.

Purification: Affinity chromatography purified via peptide column

Immunogen: Human EndoG Peptide

Isotype: IgG

Application Details

Application Notes: ELISA. Western 1 to 2 μg/mL. HepG2 and 3T3 cell lysates can be used as positive control and a 35 kDa band can be detected. Immunohistochemistry.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Buffer: PBS containing 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store the antibody undiluted at 2-8 °C.

Target Details for Endonuclease G

Target: Endonuclease G (ENDOG)

Alternative Name: Endonuclease G

Background: The fragmentation of nuclear DNA is a hallmark of apoptotic cell death. The activities of caspase and nuclease are involved in the DNA fragmentation. Caspase-activated deoxyribonuclease (CAD), also termed DNA fragmentation factor (DFF40), is one such nuclease, and is capable of inducing DNA fragmentation and chromatin condensation after cleavage by caspase-3 of its inhibitor ICAD/DFF45. Caspase and CAD independent DNA fragmentation also exists. Recent studies demonstrated that another nuclease, endonuclease G (endoG), is specifically activated by apoptotic stimuli and is able to induce nucleosomal fragmentation of DNA independently of caspase and DFF/CAD (1,2). EndoG is a mitochondrion-specific nuclease that translocates to the nucleus and cleaves chromatin DNA during apoptosis. The homologue of mammalian EndoG is the first mitochondrial protein identified to be involved in apoptosis in C. elegans (2). EndooG also cleaves DNA in vitro (4).Synonyms: ENDOG

Gene ID: 2021

NCBI Accession: NP_004426

UniProt: Q14249

Pathways: Apoptosis