GOLPH3 antibody (N-Term)

Catalog No. ABIN499904

The Rabbit Polyclonal anti-GOLPH3 antibody has been validated for WB and EIA. It is suitable to detect GOLPH3 in samples from Human, Mouse and Rat.

Quick Overview for GOLPH3 antibody (N-Term) (ABIN499904)

Target: GOLPH3 (Golgi phosphoprotein 3 (Coat-Protein) (GOLPH3))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GOLPH3 antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Product Details anti-GOLPH3 Antibody

Binding Specificity: N-Term

Purification: Affinity chromatography purified via peptide column

Immunogen: GOLPH3 antibody was raised against a 14 amino acid peptide from near the amino terminus of human GOLPH3.

Isotype: IgG

Application Details

Application Notes: ELISA. Western Blot: 0.5 - 1 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS containing 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

Storage Comment: Store the antibody (in aliquots) at -20 °C.

Target Details for GOLPH3

Target: GOLPH3 (Golgi phosphoprotein 3 (Coat-Protein) (GOLPH3))

Alternative Name: Golgi Phosphoprotein 3 / GOLPH3

Background: GOLPH3 was initially identified as a peripheral membrane protein localized to the trans-Golgi network, but others reported it to be primarily a mitochondrial protein that regulated the mitochondrial mass through the regulation of the mitochondria-specific phospholipid cardiolipin. GOLPH3 has since been implicated in the target of rapamycin (TOR) signalling pathway. Its overexpression in transfected cells led to and increase in anchorage-independent growth and cell proliferation in vitro. Furthermore, GOLPH3-transfected cells enhanced S6 Kinase activity in response to growth factor stimulation by EGF. Simultaneously, AKT phosphorylation increased in these cells, while these events were abrogated in GOLPH3 siRNA treated cells compared to control cells, indicating the GOLPH3 can enhance signalling through TOR-associated complexes. These results suggest that GOLPH3 is a bona fide oncogene and may be a useful target for therapeutic strategies.Synonyms: Coat protein GPP34, GPP34, MIDAS, Mitochondrial DNA absence factor

Gene ID: 64083

UniProt: Q9H4A6