IRF3 antibody (Intermediate Domain)

Catalog No. ABIN500056

This Rabbit Polyclonal antibody specifically detects IRF3 in WB, IF and EIA. It exhibits reactivity toward Human.

Quick Overview for IRF3 antibody (Intermediate Domain) (ABIN500056)

Target: IRF3 (Interferon Regulatory Factor 3 (IRF3))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This IRF3 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Enzyme Immunoassay (EIA)

Product Details anti-IRF3 Antibody

Binding Specificity: Intermediate Domain

Specificity: IRF3 antibody antibody was raised against a peptide corresponding to 14 amino acids near the center of human IRF3.

Purification: Affinity chromatography purified via peptide column

Immunogen: Human IRF3 (Intermediate Domain) Peptide

Isotype: IgG

Application Details

Application Notes: ELISA. Western Blot: 1 - 2 μg/mL. Immunocytochemistry.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Buffer: PBS containing 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store the antibody undiluted at 2-8 °C.

Target Details for IRF3

Target: IRF3 (Interferon Regulatory Factor 3 (IRF3))

Alternative Name: IRF3

Background: Interferons (IFN)s are involved in a multitude of immune interactions during viral infections and play a major role in both the induction and regulation of innate and adaptive antiviral mechanisms (1 for review). During infection, host-virus interactions signal downstream molecules such as transcription factors such as IFN regulatory factor-3 (IRF3) which can act to stimulate transcription of IFN-alpha/beta genes. IRF3 is present in an inactive form in the cytoplasm of most cells (2). Following viral infection, IRF3 can be activated by IkappaB kinase-epsilon and TANK-binding kinase 1 (TBK1) (3,4), whereupon IRF3 translocates to the nucleus. IRF3 can also be activated by stimulation of toll-like receptor 3 (TLR3) by dsRNA (3,5). IRF3 exists as at least two distinct isoforms.Synonyms: IRF-3, Interferon regulatory factor 3

Gene ID: 3661

NCBI Accession: NP_001562

UniProt: Q14653

Pathways: TLR Signaling, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Hepatitis C, Toll-Like Receptors Cascades