KLHL1 antibody (N-Term)

Catalog No. ABIN500126

The Rabbit Polyclonal anti-KLHL1 antibody has been validated for WB, IHC (p) and EIA. It is suitable to detect KLHL1 in samples from Human and Rat.

Quick Overview for KLHL1 antibody (N-Term) (ABIN500126)

Target: KLHL1 (Kelch-Like 1 (KLHL1))

Reactivity: Human, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This KLHL1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Product Details anti-KLHL1 Antibody

Binding Specificity: N-Term

Specificity: This antibody detects Kelch-like 1 protein.

Cross-Reactivity (Details): Species reactivity (tested):Human, rat

Purification: Peptide affinity chromatography

Immunogen: KLHL1 antibody was raised against a 14 amino acid peptide from near the amino-terminus of human KLHL1.

Isotype: IgG

Application Details

Application Notes: ELISA. Western blot: 2 - 4 μg/mL. Human brain cell lysate can be used as positive control. Immunohistochemistry on paraffin sections.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Buffer: PBS containing 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C for longer.

Target Details for KLHL1

Target: KLHL1 (Kelch-Like 1 (KLHL1))

Background: The mammalian Kelch-like 1 (KLHL1) was initially discovered as a homolog to the Drosophila Kelch gene that is highly expressed in several brain tissues (1,2). The predicted protein domain structure of KLHL1 is characteristic of a number of proteins that bind actin, form dimers, and often act as actin-organizing proteins (3). Based on the presence of anti-sense RNA that spans the transcription and translation start sites as well as the first splice site of KLHL1 in brain tissue of individuals suffering from the neurodegenerative disorder spinocerebellar ataxia type 8 (SCA8), it has been suggested that KLHL1 is involved this disease and that regulation of KLHL1 protein may be affected by antisense RNA expression (1).Synonyms: KIAA1490, KLHL1

Gene ID: 57626

UniProt: Q9NR64