Dynamin 1 antibody (AA 551-680) (AbBy Fluor® 680)

Catalog No. ABIN5001581

This anti-Dynamin 1 antibody is a Rabbit Polyclonal antibody detecting Dynamin 1 in WB, IF (cc) and IF (p). Suitable for Human.

Quick Overview for Dynamin 1 antibody (AA 551-680) (AbBy Fluor® 680) (ABIN5001581)

Target: Dynamin 1 (DNM1)

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Dynamin 1 antibody is conjugated to AbBy Fluor® 680

Application: Western Blotting (WB), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p))

Product Details anti-Dynamin 1 Antibody (AbBy Fluor® 680)

Binding Specificity: AA 551-680

Predicted Reactivity: Human,Mouse,Rat,Dog,Sheep,Pig,Horse,Chicken

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human Dynamin 1

Isotype: IgG

Application Details

Application Notes: IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for Dynamin 1

Target: Dynamin 1 (DNM1)

Alternative Name: Dynamin 1

Background:

Synonyms: B dynamin, D100, DNM 1, DNM, DNM1, DYN1_HUMAN, Dynamin, Dynamin-1, Dynamin1.

Background: Dynamin I is a GTPase enzyme required for the retrieval of synaptic vesicles after exocytosis and functions in endocytosis by stimulating assembly of invaginating synaptic vesicles (1). Dynamin I is phosphorylated in nerve terminals exclusively in the cytosolic compartment and in vitro by protein kinase C (PKC) (25). The phosphorylation site in PKC-phosphorylated Dynamin I is a single site at Serine 795, which is located near a binding site for the SH3 domain of p85, the regulatory subunit of phosphatidylinositol 3-kinase (25). Dephosphorylation is required for synaptic vesicle retrieval, suggesting that phosphorylation affects the subcellular localization of Dynamin I (5). Mouse, rat and human Dynamin I are phosphorylated on serine residues, including Ser 778, by Cdk5, regulating PACSIN1 recruitment and enabling synaptic vesicle endocytosis.

Gene ID: 1759

Pathways: Toll-Like Receptors Cascades, CXCR4-mediated Signaling Events, Thromboxane A2 Receptor Signaling