Rubella Virus Glycoprotein E1 (AA 801-900) antibody (AbBy Fluor® 750)

Catalog No. ABIN5001630

This Rabbit Polyclonal antibody specifically detects Rubella Virus Glycoprotein E1 in IF (cc) and IF (p). It exhibits reactivity toward Rubella Virus (RuV) and Virus.

Quick Overview for Rubella Virus Glycoprotein E1 (AA 801-900) antibody (AbBy Fluor® 750) (ABIN5001630)

Target: Rubella Virus Glycoprotein E1

Reactivity: Rubella Virus (RuV), Virus

Host: Rabbit

Clonality: Polyclonal

Conjugate: AbBy Fluor® 750

Application: Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p))

Product Details

Binding Specificity: AA 801-900

Cross-Reactivity: Virus

Cross-Reactivity (Details): Rubella virus

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from Rubella Virus E1 envelope glycoprotein

Isotype: IgG

Application Details

Application Notes: IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: Rubella Virus Glycoprotein E1

Alternative Name: E1 glycoprotein

Target Type: Viral Protein

Background:

Synonyms: E1, E1 envelope glycoprotein, Glycoprotein E1, Spike glycoprotein E1, POLS_RUBVM.

Background: Rubella virus (RV), the sole member of the genus Rubivirus within the family Togaviridae, is a small enveloped, positive strand RNA virus. The nucleocapsid consists of 40S genomic RNA and a single species of capsid protein which is enveloped within a host-derived lipid bilayer containing two viral glycoproteins, E1 (58 kDa) and E2 (42-46 kDa). In virus infected cells, RV matures by budding either at the plasma membrane, or at the internal membranes depending on the cell type and enters adjacent uninfected cells by a membrane fusion process in the endosome, directed by E1-E2 heterodimers. The heterodimer formation is crucial for E1 transport out of the endoplasmic reticulum to the Golgi and plasma membrane. In RV E1, a cysteine at position 82 is crucial for the E1-E2 heterodimer formation and cell surface expression of the two proteins. E1 has been shown to be a type 1 membrane protein, rich in cysteine residues with extensive intramolecular disulphide bonds [PMID: 11682134]