LKB1 antibody (C-Term)

Catalog No. ABIN500184

This anti-LKB1 antibody is a Rabbit Polyclonal antibody detecting LKB1 in WB and EIA. Suitable for Human and Mouse.

Quick Overview for LKB1 antibody (C-Term) (ABIN500184)

Target: LKB1 (STK11) (serine/threonine Kinase 11 (STK11))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This LKB1 antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Product Details anti-LKB1 Antibody

Binding Specificity: C-Term

Specificity: This antibody detects STK11.

Cross-Reactivity (Details): Species reactivity (tested):Human, mouse

Purification: Peptide affinity chromatography

Immunogen: LKB1 antibody was raised against a 15 amino acid peptide from near the carboxy terminus of human LKB1.

Isotype: IgG

Application Details

Application Notes: ELISA. Western blot: 0.5 - 1 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Buffer: PBS containing 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C for longer.

Target Details for LKB1

Target: LKB1 (STK11) (serine/threonine Kinase 11 (STK11))

Alternative Name: STK11

Background: The LKB1 serine/threonine protein kinase was initially identified as a tumor suppressor gene mutated in human Peutz-Jeghers syndrome (PJS), a condition resulting in the growth of numerous intestinal polyps classed as hamartomas. LKB1 exists as a heterotrimeric complex with two other proteins, Ste20-related adaptor protein (STRAD) and MO25. Together, this complex can phsophorylate and activate the AMP-activate protein kinase (AMPK). Following AMPK activation by LKB1, AMPK then phosphorylates TSC1 and TSC2, key components of the metabolism-regulating TOR signaling pathway, which antagonizes the activation for the TOR pathway. LKB1 has also been shown to play a fundamental role in controlling the spatial orientation of structures required to maintain an ordered, polarized epithelium. LKB1 often migrates at a higher than expected molecular weight in SDS-PAGE.Synonyms: LKB1, NY-REN-19, PJS, Renal carcinoma antigen NY-REN-19, Serine/threonine-protein kinase 11, Serine/threonine-protein kinase LKB1

Gene ID: 6794

UniProt: Q15831

Pathways: AMPK Signaling, Carbohydrate Homeostasis, Regulation of Carbohydrate Metabolic Process, Warburg Effect