EN2 antibody (AA 281-333) (AbBy Fluor® 750)

Catalog No. ABIN5001923

This anti-EN2 antibody is a Rabbit Polyclonal antibody detecting EN2 in WB, IF (cc) and IF (p). Suitable for Human and Mouse.

Quick Overview for EN2 antibody (AA 281-333) (AbBy Fluor® 750) (ABIN5001923)

Target: EN2 (Engrailed Homeobox 2 (EN2))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This EN2 antibody is conjugated to AbBy Fluor® 750

Application: Western Blotting (WB), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p))

Product Details anti-EN2 Antibody (AbBy Fluor® 750)

Binding Specificity: AA 281-333

Cross-Reactivity: Human, Mouse

Predicted Reactivity: Rat,Dog,Sheep,Pig

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human EN2/Engrailed 2

Isotype: IgG

Application Details

Application Notes: IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for EN2

Target: EN2 (Engrailed Homeobox 2 (EN2))

Alternative Name: EN2/Engrailed 2

Background:

Synonyms: Homeobox protein engrailed-2, Homeobox protein en-2, Hu-En-2, EN2

Background: The engrailed-2 gene, EN2, a murine homolog of the Drosophila homeobox gene engrailed (EN), is required for midbrain and cerebellum development and dorsal/ventral patterning of the limbs as well as apical ectodermal ridge formation. In Drosophila, the EN gene plays an important role during development in segmentation, where it is required for the formation of posterior compartments. Human EN-1 and EN-2 are homeodomain-containing proteins and have been implicated in the control of pattern formation during development of the central nervous system. Different mutations in the mouse homologs, EN-1 and EN-2, produce different developmental defects that frequently are lethal. EN-1 is highly expressed by essentially all dopaminergic neurons in the substantia nigra and ventral tegmentum. EN-1 and EN-2 regulate expression of a-synuclein, a gene that is genetically linked to Parkinson?s disease. During early brain development mouse EN-2 is expressed in a broad band across most of the mid-hindbrain region. EN-2 is also expressed in mouse myoblasts and has been assiciated with cerebellar hypoplasia.

Gene ID: 2020

UniProt: P19622