Exonuclease 1 antibody (AA 31-130) (AbBy Fluor® 750)

Catalog No. ABIN5002215

This Rabbit Polyclonal antibody specifically detects Exonuclease 1 in WB, FACS, IF (cc) and IF (p). It exhibits reactivity toward Human and Mouse.

Quick Overview for Exonuclease 1 antibody (AA 31-130) (AbBy Fluor® 750) (ABIN5002215)

Target: Exonuclease 1 (EXO1)

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Exonuclease 1 antibody is conjugated to AbBy Fluor® 750

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p))

Product Details anti-Exonuclease 1 Antibody (AbBy Fluor® 750)

Binding Specificity: AA 31-130

Cross-Reactivity: Human, Mouse

Predicted Reactivity: Rat

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human Exonuclease 1

Isotype: IgG

Application Details

Application Notes: FCM 1:20-100
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for Exonuclease 1

Target: Exonuclease 1 (EXO1)

Alternative Name: Exonuclease 1

Background:

Synonyms: exo1, EXO1_HUMAN, ExoI, Exonuclease 1, Exonuclease I, Exonuclease1, HEX1, hExo I, hExo1, hExoI, Rad2 nuclease family member homolog of S. cerevisiae exonuclease 1.

Background: Comparative evaluation of the expression patterns of the human and mouse genes, combined with previous biochemical and yeast genetic studies, indicate that the Exo1 (Exonuclease I) proteins are important contributors to chromosome processing during mammalian DNA repair and recombination. In mice, the Exo1 gene maps to distal chromosome 1, consistent with the recent mapping of the orthologous human HEX1/EXO1 gene to chromosome 1q43. Exo1 is expressed prominently in testis, an area of active homologous recombination, and spleen, a prominent lymphoid tissue. In both mammalian and yeast systems, Exo1 is a 5'-3' double stranded DNA exonuclease that has previously been implicated in DNA mismatch repair (MMR). The MMR system ensures genome integrity by removing mispaired and unpaired bases that originate during replication. In humans, Exo1 interacts with MSH2 and MLH1 and has been proposed to be a redundant exonuclease in MMR. In both mammalian and yeast systems, Exo1 plays a structural role in MMR and stabilizes multiprotein complexes containing a number of MMR proteins.

Gene ID: 9156

Pathways: DNA Damage Repair, Production of Molecular Mediator of Immune Response