Nerve Growth Factor Receptor (TNFRSF16) Associated Protein 1 (NGFRAP1) (Middle Region) antibody

Catalog No. ABIN500304

The Rabbit Polyclonal anti-Nerve Growth Factor Receptor (TNFRSF16) Associated Protein 1 antibody (ABIN500304) specifically detects Nerve Growth Factor Receptor (TNFRSF16) Associated Protein 1 in WB, IHC (p) and EIA. The antibody is reactive with Human and Mouse samples.

Quick Overview for Nerve Growth Factor Receptor (TNFRSF16) Associated Protein 1 (NGFRAP1) (Middle Region) antibody (ABIN500304)

Target: Nerve Growth Factor Receptor (TNFRSF16) Associated Protein 1 (NGFRAP1)

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Product Details

Binding Specificity: Middle Region

Specificity: This antibody detects NADE / BEX3.

Cross-Reactivity (Details): Species reactivity (tested):Human, mouse

Purification: Ion exchange chromatography

Immunogen: NADE antibody was raised against a peptide corresponding to 14 amino acids near the middle of human NADE.

Isotype: IgG

Application Details

Application Notes: ELISA. Western blot: 0.5 to 2 μg/mL. Despite its predicted molecular weight, NADE migrates at approx. 23 kDa in SDS-PAGE. Immunohistochemistry on paraffin sections.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Buffer: PBS containing 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C for longer.

Target Details

Target: Nerve Growth Factor Receptor (TNFRSF16) Associated Protein 1 (NGFRAP1)

Alternative Name: NADE / BEX3

Background: The p75 neurotrophin receptor (p75NTR) is a member of the tumor necrosis receptor superfamily and can mediate cell death and cell survival in response to nerve growth factor (NGF) (1 for review). The p75NTR-associated cell death executor (NADE) mediates apoptosis by interacting with the cell death domain of p75NTR following the binding of NGF by p75NTR (2). Recent studies have shown that NADE also interacts with second mitochondria-derived activator of caspase (Smac). Co-expression of NADE and Smac promotes TRAIL-induced apoptosis and inhibits XIAP-mediated Smac ubiquitization. It has been suggested that it is this interaction between NADE and Smac that allows apoptosis to proceed (5). Finally, although initially discovered as an mRNA expressed in ovarian granulosa cells (3), NADE has been suggested to play a role in the neuronal death seen in epileptic brain damage (4).Synonyms: Brain-expressed X-linked protein 3, DXS6984E, NGFRAP1, p75NTR-associated cell death executor

Gene ID: 27018

NCBI Accession: NP_996798

UniProt: Q00994

Pathways: Neurotrophin Signaling Pathway, Positive Regulation of Endopeptidase Activity