Nephrin antibody (C-Term)

Catalog No. ABIN500330

The Rabbit Polyclonal anti-Nephrin antibody has been validated for WB, IF, IHC (p) and EIA. It is suitable to detect Nephrin in samples from Human, Mouse and Rat. There are 2+ publications available.

Quick Overview for Nephrin antibody (C-Term) (ABIN500330)

Target: Nephrin (NPHS1) (Nephrosis 1, Congenital, Finnish Type (Nephrin) (NPHS1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Nephrin antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Product Details anti-Nephrin Antibody

Binding Specificity: C-Term

Specificity: This antibody detects Nephrin.

Purification: Peptide affinity chromatography

Immunogen: Nephrin antibody was raised against a 14 amino acid peptide from near the carboxy terminus of human Nephrin.

Isotype: IgG

Application Details

Application Notes: ELISA. Western blot: 0.5 - 1 μg/mL. Immunohistochemistry on paraffin sections.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS containing 0.02 % Sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

References for anti-Nephrin antibody (ABIN500330)

Gu, Zhou, Noble, Border, Cheung, Huang: "Targeting reduction of proteinuria in glomerulonephritis: Maximizing the antifibrotic effect of valsartan by protecting podocytes." in: Journal of the renin-angiotensin-aldosterone system : JRAAS, Vol. 15, Issue 2, pp. 177-89, (2015) (PubMed).

Zhang, Gu, Lawrence, Cheung, Huang: "A plasminogen activator inhibitor type 1 mutant retards diabetic nephropathy in db/db mice by protecting podocytes." in: Experimental physiology, Vol. 99, Issue 5, pp. 802-15, (2014) (PubMed).

Target Details for Nephrin

Target: Nephrin (NPHS1) (Nephrosis 1, Congenital, Finnish Type (Nephrin) (NPHS1))

Alternative Name: Nephrin

Background: Nephrin is strongly expressed in renal glomeruli and is a member of the immunoglobulin family of cell adhesion molecules. Mutations in the Nephrin gene result in congenital nephrotic syndrome, an autosomal-recessive disorder characterized by massive proteinuria in utero and nephrosis at birth. Renal glomeruli allow normal kidneys to filter plasma so that it is very pure. Nephrin is expressed in the podocyte slit-diaphragm of the renal glomeruli in a manner that suggests that Nephrin molecules homodimerize in an anti-parallel fashion similar to cadherin interactions in adherens junctions. Thus, Nephrin may constitute the entire extracellular structure of the slit-diaphragm. Despite its predicted molecular weight, Nephrin often migrates at a lower than expected size in SDS-PAGE.Synonyms: NPHN, NPHS1

Gene ID: 4868

NCBI Accession: NP_004637

UniProt: O60500

Pathways: Regulation of Actin Filament Polymerization, Skeletal Muscle Fiber Development