Histone H1 antibody (acLys52) (AbBy Fluor® 750)

Catalog No. ABIN5004655

The Rabbit Polyclonal anti-Histone H1 antibody has been validated for FACS, IF (cc) and IF (p). It is suitable to detect Histone H1 in samples from Human, Mouse and Rat.

Quick Overview for Histone H1 antibody (acLys52) (AbBy Fluor® 750) (ABIN5004655)

Target: Histone H1

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Histone H1 antibody is conjugated to AbBy Fluor® 750

Application: Flow Cytometry (FACS), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p))

Product Details anti-Histone H1 Antibody (AbBy Fluor® 750)

Binding Specificity: acLys52

Specificity: This antibody will react with members of the Histone H1 family that have an acetylated lysine positioned in the motif AS(Ac-K)ER. This include H1.1, H1.2, H1.3, H1.4, and H1.5.

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: Dog,Cow,Pig,Horse

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic acetylpeptide derived from human Histone H1.4 around acetylation site of Lys52

Isotype: IgG

Application Details

Application Notes: FCM 1:20-100
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for Histone H1

Target: Histone H1

Background:

Synonyms: H1E, H1.4, H1F4, H1s-4, dJ221C16.5, Histone H1.4, Histone H1b, Histone H1s-4, HIST1H1E

Background: Histone H1 protein binds to linker DNA between nucleosomes forming the macromolecular structure known as the chromatin fiber. Histones H1 are necessary for the condensation of nucleosome chains into higher-order structured fibers. Acts also as a regulator of individual gene transcription through chromatin remodeling, nucleosome spacing and DNA methylation (By similarity).

Gene ID: 3008

UniProt: P10412