ANP32E antibody (C-Term)

Catalog No. ABIN500487

This anti-ANP32E antibody is a Rabbit Polyclonal antibody detecting ANP32E in WB, IHC (p) and EIA. Suitable for Human, Mouse and Rat.

Quick Overview for ANP32E antibody (C-Term) (ABIN500487)

Target: ANP32E (Acidic (Leucine-Rich) Nuclear phosphoprotein 32 Family, Member E (ANP32E))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ANP32E antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Product Details anti-ANP32E Antibody

Binding Specificity: C-Term

Specificity: This antibody detects ANP32E / PHAP III. It has no cross-reaction to PHAP I and PHAP I2a.

Cross-Reactivity (Details): Species reactivity (tested):Human, mouse, rat

Purification: Peptide affinity chromatography

Immunogen: ANP32E antibody was raised with a synthetic peptide corresponding to amino acids close to carboxy terminus of human ANP32E / PHAP III.

Isotype: IgG

Application Details

Application Notes: ELISA. Western blot: 0.5 to 2 μg/mL, a band at approximately 35 kDa can be detected. Immunohistochemistry on paraffin sections.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: PBS containing 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody at 2-8 °C for up to one month or (in aliquots) at -20 °C for longer.

Target Details for ANP32E

Target: ANP32E (Acidic (Leucine-Rich) Nuclear phosphoprotein 32 Family, Member E (ANP32E))

Alternative Name: ANP32E

Background: Apoptosis is related to many diseases and development. Caspase-9 plays a central role in cell death induced by a variety of apoptosis activators. Cytochrome c, after released from mitochondria, binds to Apaf-1, which forms an apoptosome that in turn binds to and activate procaspase-9. Activated caspase-9 cleaves and activates the effector caspases (caspase-3, -6 and -7), which are responsible for the proteolytic cleavage of many key proteins in apoptosis. The tumor suppressor putative HLA-DR-associated proteins (PHAPs) were recently identified as important regulators of mitochondrion apoptosis (1). PHAP appears to facilitate apoptosome-medicated caspase-9 activation and to stimulate the mitochondrial apoptotic pathway. PHAP was also shown to oppose both Ras- and Myc-medicated cell transformation.Synonyms: Acidic leucine-rich nuclear phosphoprotein 32 family member E, LANP-L, LANP-like protein, LANPL, PHAP III

Gene ID: 81611

NCBI Accession: NP_112182

UniProt: Q9BTT0