HSD17B4 antibody (AA 521-620) (AbBy Fluor® 680)

Catalog No. ABIN5004883

The Rabbit Polyclonal anti-HSD17B4 antibody has been validated for WB, IF (cc) and IF (p). It is suitable to detect HSD17B4 in samples from Mouse, Sheep and Zebrafish (Danio rerio).

Quick Overview for HSD17B4 antibody (AA 521-620) (AbBy Fluor® 680) (ABIN5004883)

Target: HSD17B4 (Hydroxysteroid (17-Beta) Dehydrogenase 4 (HSD17B4))

Reactivity: Mouse, Sheep, Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HSD17B4 antibody is conjugated to AbBy Fluor® 680

Application: Western Blotting (WB), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p))

Product Details anti-HSD17B4 Antibody (AbBy Fluor® 680)

Binding Specificity: AA 521-620

Cross-Reactivity: Mouse, Sheep, Zebrafish (Danio rerio)

Predicted Reactivity: Human,Rat,Cow,Pig,Horse,Chicken,Guinea Pig

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human HSD17B4 Enoyl-CoA hydratase 2

Isotype: IgG

Application Details

Application Notes: IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for HSD17B4

Target: HSD17B4 (Hydroxysteroid (17-Beta) Dehydrogenase 4 (HSD17B4))

Alternative Name: HSD17B4

Background:

Synonyms: hydroxysteroid 17-beta dehydrogenase 4, 12-alpha-trihydroxy-5-beta-cholest-24-enoyl-CoA hydratase, 17 beta HSD 4, 17 beta HSD IV, 17 beta hydroxysteroid dehydrogenase 4, 17-beta-HSD 4, 17-beta-hydroxysteroid dehydrogenase 4, 17beta estradiol dehydrogenase type IV, 3 alpha 7 alpha12 alpha trihydroxy 5 beta cholest 24 enoyl CoA hydratase antibody 3-alpha, 7-alpha, Beta hydroxyacyl dehydrogenase, Beta keto reductase, D 3 hydroxyacyl CoA dehydratase, D bunctional protein, D bunctional protein peroxisomal, D-bunctional protein, DBP, DHB4_HUMAN, EDH17B4, Enoyl-CoA hydratase 2, Hsd17b4, MFE 2, MFE-2, MPF-2, Multunctional protein 2, Peroxisomal multunctional enzyme type 2, Peroxisomal multunctional protein 2, SDR8C1, Short chain dehydrogenase/reductase family 8C member 1.

Background: 17Beta-HSD4 (17Beta-hydroxysteroid dehydrogenase type 4) is also known as peroxisomal multifunctional enzyme/protein 2 (MFE-2/MFP-2), D-bifunctional enzyme or 17-Beta Estradiol dehydrogenase type IV. It belongs to the 17Beta-HSD family of proteins that regulate the availability of steroids within various tissues throughout the body. 17Beta-HSD4 inactivates Estradiol through its oxidative activity but it is primarily involved in peroxisomal fatty acid and cholesterol Beta-oxidation. It has a multi-domain structure: the dehydrogenase domain is fused to a hydratase and a lipid transfer domain. 17Beta-HSD4 is a target protein of chromeceptin and it is essential for the downstream activation of Stat6. 17Beta-HSD4-deficient patients exhibit Zellweger-like syndrome and die within the first year of life. They display neuronal migration defects, facial dysmorphisms, severe hypotonia and convulsions in the neonatal period.

Pathways: Monocarboxylic Acid Catabolic Process