PPPDE1 antibody (C-Term)

Catalog No. ABIN500519

The Rabbit Polyclonal anti-PPPDE1 antibody has been validated for WB and EIA. It is suitable to detect PPPDE1 in samples from Human, Mouse and Rat.

Quick Overview for PPPDE1 antibody (C-Term) (ABIN500519)

Target: PPPDE1 (PPPDE Peptidase Domain Containing 1 (PPPDE1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PPPDE1 antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Product Details anti-PPPDE1 Antibody

Binding Specificity: C-Term

Specificity: This antibody detects PNAS4.

Cross-Reactivity (Details): Species reactivity (tested):Human, mouse, rat

Purification: Peptide affinty chromatography

Immunogen: PNAS4 antibody was raised against a 19 amino acid peptide near the carboxy terminus of human PNAS4.

Isotype: IgG

Application Details

Application Notes: ELISA. Western blot: 1 - 2 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Buffer: PBS containing 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C for longer.

Target Details for PPPDE1

Target: PPPDE1 (PPPDE Peptidase Domain Containing 1 (PPPDE1))

Alternative Name: PPPDE1

Background: PNAS4 is a highly conserved protein that shares high homology from plants to animals. It was initially identified as a putative apoptosis-related protein in the human acute promyelocytic leukemia cell line NB4. PNAS4 is activated during the early response to DNA damage and can inhibit proliferation via apoptosis when overexpressed in some tumor cells such as U2OS, SKOV3, and A549. PNAS4 inhibits tumor cell proliferation through the following mechanisms: (i) overexpression of PNAS4 causes S phase arrest by regulating the expression of cell cycle-related proteins and (ii) PNAS4 induces apoptosis through the mitochondrial apoptosis pathway. Recent evidence has shown that PNAS4 may be involved in the genesis of some cancers and could be a potential candidate for lung cancer therapy alone or in combination with gemcitabine. At least two isoforms of PNAS4 are known to exist.Synonyms: C1orf121, CGI-146, FAM152A, PNAS-4, PNAS4, PPPDE peptidase domain-containing protein 1

Gene ID: 51029

NCBI Accession: NP_057160

UniProt: Q9BSY9