PPPDE1 antibody (Internal Region)

Catalog No. ABIN5539905

Product Details anti-PPPDE1 Antibody

Target: PPPDE1 (PPPDE Peptidase Domain Containing 1 (PPPDE1))

Binding Specificity: Internal Region

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This PPPDE1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Purpose: PPPDE1 / PNAS4

Sequence: ELGKEYKGNA YH

Cross-Reactivity: Cow, Dog, Human, Mouse, Pig, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Grade: Verified

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: Paraffin embedded Human Skin. Recommended concentration: 10 μg/mL.

Western Blot: In transfected HEK293 transiently expressing full-length Human PPPDE1(myc and DYKDDDDK tagged), a band of approx. 30 kDa was observed. No bands were observed in mock-transfected HEK293 and the same band was observed using anti-myc tag antibo

Peptide ELISA: antibody detection limit dilution 1:32000.

Comment:

Purchase the +ve control! The transfected lysate used to QC this product is available for sale. Please email sales@everestbiotech.com to request datasheet, pricing and delivery information.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for PPPDE1

Target: PPPDE1 (PPPDE Peptidase Domain Containing 1 (PPPDE1))

Alternative Name: DESI2 (PPPDE1 Products)

Background: PPPDE1, PPPDE peptidase domain containing 1, C1orf121, CGI-146, FAM152A, FLJ21998, PNAS-4, family with sequence similarity 152, member A

Gene ID: 51029, 78825, 289277

NCBI Accession: NP_057160