RGS21 antibody (Center)

Catalog No. ABIN500587

This Rabbit Polyclonal antibody specifically detects RGS21 in WB, IHC (p) and EIA. It exhibits reactivity toward Human.

Quick Overview for RGS21 antibody (Center) (ABIN500587)

Target: RGS21 (Regulator of G-Protein Signaling 21 (RGS21))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RGS21 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Product Details anti-RGS21 Antibody

Binding Specificity: Center

Specificity: This antibody detects RGS21.

Cross-Reactivity (Details): Species reactivity (tested):Human.

Purification: Peptide Affinity Chromatography

Immunogen: RGS21 antibody was raised against a 16 amino acid peptide near the center of Human RGS21.

Isotype: IgG

Application Details

Application Notes: ELISA. Western blot: 1-2 μg/mL (HepG2 cells as recommended Positive Control). Immunocytochemistry.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS containing 0.02 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for RGS21

Target: RGS21 (Regulator of G-Protein Signaling 21 (RGS21))

Alternative Name: RGS21

Background: Regulator of G-protein signaling (RGS) proteins contain an 120 amino acid conserved domain, termed the RGS domain, that acts as a GTPase-activating protein that acts to reduce the signal transmitted by the receptor-activated G-alpha subunit. RGS21 is a recently identified member of this family that has been reported to be selectively expressed in subpopulations of taste bud cells and co-expressed with bitter and sweet transduction components such as alpha-gusticin, phospholipase Cbeta2, T1R2/T1R3 sweet taste receptors and T2R bitter taste receptors. Other reports indicate that RGS21 is more widely expressed. Binding assays demonstrate that RGS21 binds alpha-gusticin in a conformation-dependent manner and may do the same with T1R receptors, suggesting that RGS21 may play a role in sweet and bitter taste transduction processes.Synonyms: Regulator of G-protein signaling 21

Gene ID: 431704

NCBI Accession: NP_001034241

UniProt: Q2M5E4

Pathways: Regulation of G-Protein Coupled Receptor Protein Signaling