RSPO1 antibody (N-Term)

Catalog No. ABIN500638

This Rabbit Polyclonal antibody specifically detects RSPO1 in WB, IHC (p) and EIA. It exhibits reactivity toward Human, Rat and Mouse.

Quick Overview for RSPO1 antibody (N-Term) (ABIN500638)

Target: RSPO1 (R-Spondin 1 (RSPO1))

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RSPO1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Product Details anti-RSPO1 Antibody

Binding Specificity: N-Term

Specificity: This antibody detects RSPO1. It will not cross-react with RSPO family members.

Cross-Reactivity (Details): Species reactivity (tested):Human, mouse, rat

Purification: Peptide affinity chromatography

Immunogen: RSPO1 antibody was raised against a 16 amino acid peptide from near the amino terminus of human RSPO1.

Isotype: IgG

Application Details

Application Notes: ELISA. Western blot: 1 - 2 μg/mL. Immunohistochemistry on paraffin sections.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Buffer: PBS containing 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C for longer.

Target Details for RSPO1

Target: RSPO1 (R-Spondin 1 (RSPO1))

Alternative Name: RSPO1

Background: RSPO1 is a member of a family of secreted growth factors that can operate through the canonical Wnt signaling pathway by stabilizing the intracellular b-catenin, thereby regulating functions mediated by b-catenin such as cell fate decisions and embryonic patterning. RSPO1 was recently identified through linkage analysis to be involved in sex determination and mammalian ovarian development. RSPO1 is thought to regulate cellular responsiveness to Wnt ligands by modulating the cell-surface expression of the Wnt co-receptor LRP6 by interfering with the DKK/Kremen-mediated internalization of LRP6 through an interaction with Kremen, resulting in increased LRP6 cell-surface levels. At least two isoforms of RSPO1 are known to exist.Synonyms: R-spondin-1, Roof plate-specific spondin-1

Gene ID: 284654

NCBI Accession: NP_001033722

UniProt: Q2MKA7