SIRPA antibody (C-Term)

Catalog No. ABIN500722

This Rabbit Polyclonal antibody specifically detects SIRPA in WB, IF and EIA. It exhibits reactivity toward Human, Rat and Mouse.

Quick Overview for SIRPA antibody (C-Term) (ABIN500722)

Target: SIRPA (Signal-Regulatory Protein alpha (SIRPA))

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SIRPA antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Enzyme Immunoassay (EIA)

Product Details anti-SIRPA Antibody

Binding Specificity: C-Term

Specificity: This antibody is directed against CD172a / SIRPA C-term. 75-110 kDa bands should be detected and it recognizes SIRP alpha 1, 2 and 3.

Cross-Reactivity (Details): Species reactivity (tested):Human, Mouse, Rat.

Purification: DEAE Chromatography

Immunogen: SIRP alpha antibody was raised against a peptide corresponding to amino acids near the carboxy terminus of human SIRP alpha 1. .Remarks: The sequences of the immunogenic peptide differ from those of Mouse, Rat and Bovine by one amino acid.

Isotype: IgG

Application Details

Application Notes: ELISA. Western blot (0.5-1 μg/mL). Whole cell lysate from THP-1 cells can be used as positivecontrol. Immunoflourescence.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS, 0.02 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for SIRPA

Target: SIRPA (Signal-Regulatory Protein alpha (SIRPA))

Alternative Name: CD172a / SIRPA

Background: Protein tyrosine phosphatases (PTPases) SHP-1 and SHP-2 are critical regulators in the intracellular signaling pathways that result in cell responses such as mitosis, differentiation, migration, survival, transformation or death. SHP-2 is a signal transducer for several receptor tyrosine kinases and cytokine receptors. A novel SHP-2 associated glycoprotein was recently cloned from human, rat, mouse and cattle by several labs and was designated SIRPalpha, SHPS-1, MyD-1, BIT and p84. SIRPalpha is a new gene family containing at least fifteen members. SIRPalpha is a substrate of many activated tyrosine kinases such as insulin receptor, EGFR, PDGFR and src, and a specific docking protein for SHP-2. SIRPalpha has regulatory effects on cellular responses induced by serum, growth factors, insulin, oncogenes, growth hormones and cell adhesion and plays a general role in different physiological and pathological processes.Synonyms: BIT, Brain Ig-like molecule with tyrosine-based activation motifs, CD172 antigen-like family member A, Inhibitory receptor SHPS-1, MFR, MYD1, Macrophage fusion receptor, MyD-1 antigen, PTPNS1, SHP substrate 1, SHPS-1, SHPS1, Signal-regulatory protein alpha-1, Sirp-alpha-2, Sirp-alpha-3, Tyrosine-protein phosphatase non-receptor type substrate 1, p84

Molecular Weight: 75-110 kDa

Gene ID: 140885

NCBI Accession: NP_542970

UniProt: P78324