NLE1 antibody (AA 351-450) (AbBy Fluor® 680)

Catalog No. ABIN5007676

The Rabbit Polyclonal anti-NLE1 antibody has been validated for WB, IF (cc) and IF (p). It is suitable to detect NLE1 in samples from Mouse.

Quick Overview for NLE1 antibody (AA 351-450) (AbBy Fluor® 680) (ABIN5007676)

Target: NLE1 (Notchless Homolog 1 (NLE1))

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NLE1 antibody is conjugated to AbBy Fluor® 680

Application: Western Blotting (WB), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p))

Product Details anti-NLE1 Antibody (AbBy Fluor® 680)

Binding Specificity: AA 351-450

Cross-Reactivity: Mouse

Predicted Reactivity: Human,Rat,Cow,Sheep,Pig,Horse,Rabbit

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human NLE1

Isotype: IgG

Application Details

Application Notes: IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for NLE1

Target: NLE1 (Notchless Homolog 1 (NLE1))

Alternative Name: NLE1

Background:

Synonyms: FLJ10458, NLE 1, Nle, Notchless gene homolog, Notchless homolog 1 Drosophila, Notchless homolog 1, Notchless protein homolog 1, NLE1_HUMAN.

Background: The Notch signaling pathway is an evolutionary conserved system that is involved in intracellular communication. Notch receptors play an important role in development and cell-fate decisions. Notchless is a loss-of-function mutant allele that encodes for protein NLE1 (notchless homolog 1). NLE1 is a 485 amino acid WD40-repeat protein that binds to the cytoplasmic domain of Notch, regulating its signaling activity in Drosophila melanogaster and in mice. Deletion of the NLE1 gene in mice during the early stages of development results in embryonic death, while gene deletion in the late stages of development leads to activation of a caspase-3-dependent apoptotic pathway. In plants, NLE1 is crucial for normal cellular growth and development. Under-expression during shoot proliferation causes pleiotropic defects such as delayed flowering and abnormal organ maturation. It may also play a role in 60S ribosomal subunit biogenesis in yeast. NLE1 contains eight WD40 domains and produces one isoform due to alternative splicing.

Gene ID: 54475